Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine®, Carrier-free

• Background:

  Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

• :

  heparin, EDTA or citrate

• Description:

  Human IL-1 Beta/IL-1F2/IL1B ELISA Kit PicoKine® (96 Tests). Quantitate Human IL1B in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	11	10	11	11	10	0.43	4.3%
  Sample 2	60	54	52	53	54	3.11	5.7%
  Sample 3	123	119	123	112	119	4.49	3.7%

  *number of samples for each test n=16.

• Synonyms:

  Interleukin-1 beta;IL-1 beta;Catabolin;IL1B;IL1F2;

• Gene Name:

  Interleukin-1 beta

• Gene ID:

  3553

• UniProt:

  P01584

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: E.coli; Immunogen sequence: A117-S269

• Applications:

  ELISA

• Standard:

  1 ng/tube

• Sample Type:

  cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

• Detection Range:

  3.9 pg/ml - 250 pg/ml

• Sensitivity:

  <0.15 pg/ml

• Function:

  Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  30748 MW

• Specificity:

  Natural and recombinant human IL-1 beta

• Subcellular Location:

  Secreted. The lack of a specific hydrophobic segment in the precursor sequence suggests that IL-1 is released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

• Sequence Similarities:

  Belongs to the IL-1 family.

• Protein Name:

  Interleukin-1 beta