Human IGFBP-1 ELISA Kit PicoKine®, Carrier-free

• Background:

  IGFBP1 is also known as amniotic fluid binding protein (AFBP), placental protein-12, alpha-pregnancy-associated endometrial globulin, growth hormone independent binding protein, binding protein-28, binding protein-26, and binding protein-25. The low-molecular weight insulin-like growth-factor binding protein (IGF-BP25) is synthesized by human liver, secretory endometrium and decidua, and is also present in human serum. It binds insulin-like growth factors IGF-I and IGF-II with high affinity, and is proposed to act as a paracrine regulator of cell growth. These IGF-binding proteins are expressed at different concentrations in different tissues and are thought to regulate the activity of IGF I and II. The gene is organized in four exons and spans 5.9 kb. The IBP-1 gene is a single copy gene, located on chromosome 7. The standard product used in this kit is recombinant human IGFBP-1, consisting of 234 amino acids with the molecular mass of 25KDa.

• :

  heparin or EDTA

• Description:

  Human IGFBP-1 ELISA Kit PicoKine® (96 Tests). Quantitate Human IGFBP1 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.

• Reproducibility:

  Lots	Lot 1 (pg/ml)	Lot 2 (pg/ml)	Lot 3 (pg/ml)	Lot 4 (pg/ml)	Mean (pg/ml)	Standard Deviation	CV (%)
  Sample 1	66	74	68	68	69	3	4.3%
  Sample 2	243	252	252	256	250	4.76	1.9%
  Sample 3	1016	979	1037	933	991	39.52	3.9%

  *number of samples for each test n=16.

• Synonyms:

  Insulin-like growth factor-binding protein 1;IBP-1;IGF-binding protein 1;IGFBP-1;Placental protein 12;PP12;IGFBP1;IBP1;

• Gene Name:

  Insulin-like growth factor-binding protein 1

• Gene ID:

  3484

• UniProt:

  P08833

• Reactivity:

  Human

• Cross Reactivity:

  There is no detectable cross-reactivity with other relevant proteins.

• Immunogen:

  Expression system for standard: NS0; Immunogen sequence: A26-N259

• Applications:

  ELISA

• Standard:

  10 ng/tube

• Sample Type:

  cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

• Detection Range:

  31.2 pg/ml - 2,000 pg/ml

• Sensitivity:

  <1 pg/ml

• Function:

  IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration. .

• Components:

  Description|Quantity Pre-coated 96-well strip microplate|1 Standard|2 vials Biotinylated antibody (100x)|100ul Avidin-Biotin-Peroxidase Complex (100x)|100ul Sample Diluent|30ml Antibody Diluent|12ml Avidin-Biotin-Peroxidase Diluent|12ml Color Developing Reagent (TMB)|10ml Stop Solution|10ml Wash Buffer (25x)|20ml Adhesive plate sealers|4

• Storage Conditions:

  Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

• Calculated Molecular Weight:

  27904 MW

• Specificity:

  Natural and recombinant human IGFBP-1

• Subcellular Location:

  Secreted.

• Sequence Similarities:

  Contains 1 IGFBP N-terminal domain.

• Protein Name:

  Insulin-like growth factor-binding protein 1