Anti-DCP1A Monoclonal Antibody, Carrier-free

• Description:

  Boster Bio Anti-DCP1A Monoclonal Antibody catalog # M04587. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Caldesmon;CDM;CALD1;CAD, CDM;

• Gene Name:

  Caldesmon

• UniProt:

  Q9NPI6

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Immunogen:

  A synthesized peptide derived from human DCP1A Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Contributes to the transactivation of target genes after stimulation by TGFB1.

• Clonality:

  Monoclonal

• Clone:

  Clone: AEFC-4

• Tissue Specificity:

  High-molecular-weight caldesmon (isoform 1) is predominantly expressed in smooth muscles, whereas low-molecular- weight caldesmon (isoforms 2, 3, 4 and 5) are widely distributed in non-muscle tissues and cells. Not expressed in skeletal muscle or heart.

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Actin- and myosin-binding protein implicated in the regulation of actomyosin interactions in smooth muscle and nonmuscle cells (could act as a bridge between myosin and actin filaments). Stimulates actin binding of tropomyosin which increases the stabilization of actin filament structure. In muscle tissues, inhibits the actomyosin ATPase by binding to F-actin. This inhibition is attenuated by calcium-calmodulin and is potentiated by tropomyosin. Interacts with actin, myosin, two molecules of tropomyosin and with calmodulin. Also play an essential role during cellular mitosis and receptor capping. Involved in Schwann cell migration during peripheral nerve regeneration (By similarity). .

• Components:

  Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml BSA.

• Storage Conditions:

  Store at -20℃ for one year. For short term storage and frequent use, store at 4℃ for up to one month. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  93231 MW

• Observed Molecular Weight:

  52 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200
  FC 1:100

• Subcellular Location:

  Cytoplasm, cytoskeleton . Cytoplasm, myofibril . On thin filaments in smooth muscle and on stress fibers in fibroblasts (nonmuscle). .

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain