Anti-BOB1/POU2AF1 Antibody Picoband®, Carrier-free

• Background:

  POU2AF1 (POU domain class 2-associating factor 1), also known as OBF1, BOB1 and OCAB, is a protein that in humans is encoded by the POU2AF1 gene. POU domain proteins contain a bipartite DNA-binding domain divided by a flexible linker that enables them to adopt various monomer configurations on DNA. The versatility of POU protein operation is additionally conferred at the dimerization level. Casellas et al. (2002) reported that OCAB is essential for V (D)J recombination of a subset of Ig-kappa genes. They showed that OCAB modulates recombination by ly enhancing Ig-kappa gene transcription in vivo. Siegel et al. (2006) suggested that, together with dysregulation of other OCAB target genes, altered regulation of SYK may help explain the magnitude of defects observed in B-cell development, including the pre-B1-to-pre-B2 transition, and immune responses in Ocab -/- mice.

• Description:

  Boster Bio Anti-BOB1/POU2AF1 Antibody catalog # PA1506. Tested in IHC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  POU domain class 2-associating factor 1;B-cell-specific coactivator OBF-1;BOB-1;BOB1;OCA-B;OCT-binding factor 1;Pou2af1;Obf-1;

• Gene Name:

  POU domain class 2-associating factor 1

• UniProt:

  Q64693

• Host:

  Rabbit

• Reactivity:

  Rat,Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the C-terminus of mouse BOB1, different from the related rat sequence by one amino acid, and from human sequence by four amino acids.

• Clonality:

  Polyclonal

• Tissue Specificity:

  B-cell specific.

• Applications:

  IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Casellas, R., Jankovic, M., Meyer, G., Gazumyan, A., Luo, Y., Roeder, R. G., Nussenzweig, M. C.OcaB is required for normal transcription and V(D)J recombination of a subset of immunoglobulin kappa genes.Cell 110: 575-585, 2002. 2. Siegel, R., Kim, U., Patke, A., Yu, X., Ren, X., Tarakhovsky, A., Roeder, R. G.Nontranscriptional regulation of SYK by the coactivator OCA-B is required at multiple stages of B cell development.Cell 125: 761-774, 2006.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  27692 MW

• Observed Molecular Weight:

  37 kDa

• Fragment:

  Rabbit IgG

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Rat, Mouse, By Heat
  Western blot, 0.1-0.5μg/ml, Mouse
  

• Subcellular Location:

  Nucleus.

• Protein Name:

  POU domain class 2-associating factor 1