Anti-POLR3GL Antibody Picoband®, APC Conjugate

• Background:

  Polymerase (RNA) III (DNA ed) polypeptide G (32kD)-like also known as POLR3GL is a protein which in humans is encoded by the POLR3GL gene.Predicted to enable chromatin binding activity. Involved in transcription by RNA polymerase III. Located in nucleus. Part of RNA polymerase III complex.

• Description:

  Boster Bio Anti-POLR3GL Antibody Picoband® catalog # A17067-1. Tested in ELISA, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Kelch repeat and BTB domain-containing protein 2; BTB and kelch domain-containing protein 1; KBTBD2; BKLHD1; KIAA1489

• Gene Name:

  kelch repeat and BTB domain containing 2

• Gene ID:

  84265

• UniProt:

  Q9BT43

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human POLR3GL recombinant protein (Position: R8-K156).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in liver, skeletal muscle, kidney, pancreas, spleen, thyroid, testis, ovary, small intestine and colon.

• Applications:

  ELISA,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Required for DNA double-strand breaks (DSBs) formation in unsynapsed regions during meiotic recombination. Probably acts by forming a complex with MEI4 and REC114, which activates DSBs formation in unsynapsed regions, an essential step to ensure completion of synapsis. Not required for HORMAD1 functions in pairing-independent synaptonemal complex formation, ATR recruitment to unsynapsed axes, meiotic silencing of unsynapsed chromatin (MSUC) or meiotic surveillance.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Beauregard-Lacroix, E., Salian, S., Kim, H., Ehresmann, S., D'Amours, G., Gauthier, J., Saillour, V., Bernard, G., Mitchell, G. A., Soucy, J. F., Michaud, J. L., Campeau, PM. A variant of neonatal progeroid syndrome, or Wiedemann-Rautenstrauch syndrome, is associated with a nonsense variant in POLR3GL. Europ. J. Hum. Genet. 28: 461-468, 2020. 2. Haurie, V., Durrieu-Gaillard, S., Dumay-Odelot, H., Da Silva, D., Rey, C., Prochazkova, M., Roeder, R. G., Besser, D., Teichmann, M. Two isoforms of human RNA polymerase III with specific functions in cell growth and transformation. Proc. Nat. Acad. Sci. 107: 4176-4181, 2010. 3. Renaud, M., Praz, V., Vieu, E., Florens, L., Washburn, M. P., l'Hote, P., Hernandez, N. Gene duplication and neofunctionalization: POLR3G and POLR3GL. Genome Res. 24: 37-51, 2014.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  24145 MW

• Observed Molecular Weight:

  24 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cul3-RING ubiquitin ligase complex.

• Protein Name:

  Transmembrane protein 240