Anti-BI-1/TMBIM6 Antibody Picoband®, APC Conjugate

• Background:

  Bax inhibitor 1 is a protein that in humans is encoded by the TMBIM6 gene. Enables endoribonuclease inhibitor activity and ubiquitin protein ligase binding activity. Involved in several processes, including negative regulation of RNA metabolic process; negative regulation of intrinsic apoptotic signaling pathway; and response to L-glutamate. Acts upstream of or within negative regulation of calcium ion transport into cytosol. Located in endoplasmic reticulum membrane and mitochondrial membrane. Biomarker of cervical squamous cell carcinoma and prostate carcinoma.

• Description:

  Boster Bio Anti-BI-1/TMBIM6 Antibody Picoband® catalog # A05462. Tested in IF, IHC, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Prion-like protein doppel; PrPLP; Prion protein 2; PRND; DPL; UNQ1830/PRO3443

• Gene Name:

  prion like protein doppel

• Gene ID:

  7009

• UniProt:

  P55061

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human BI-1/TMBIM6, identical to the related mouse and rat sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in testis, in Sertoli cells, ejaculated spermatozoa and in seminal fluid (at protein level).

• Applications:

  IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Required for normal acrosome reaction and for normal male fertility (By similarity). Can bind Cu (2+) (PubMed:15218028, PubMed:20411530).

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Bailly-Maitre, B., Fondevila, C., Kaldas, F., Droin, N., Luciano, F., Ricci, J.-E., Croxton, R., Krajewska, M., Zapata, J. M., Kupiec-Weglinski, J. W., Farmer, D., Reed, J. C. Cytoprotective gene bi-1 is required for intrinsic protection from endoplasmic reticulum stress and ischemia-reperfusion injury. Proc. Nat. Acad. Sci. 103: 2809-2814, 2006. 2. Carrara, G., Saraiva, N., Gubser, C., Johnson, B. F., Smith, G. L. Six-transmembrane topology for Golgi anti-apoptotic protein (GAAP) and Bax inhibitor 1 (BI-1) provides model for the transmembrane Bax inhibitor-containing motif (TMBIM) family. J. Biol. Chem. 287: 15896-15905, 2012. 3. Gross, M. B. Personal Communication. Baltimore, Md. 3/22/2016.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  39411 MW

• Observed Molecular Weight:

  35 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml/ml, Human
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Immunofluorescence, 5 μg/ml, Human
  

• Subcellular Location:

  Cell membrane.

• Protein Name:

  Zinc finger protein Helios