Anti-PPAN Antibody Picoband®, FITC Conjugate

• Background:

  Suppressor of SWI4 1 homolog is a protein that in humans is encoded by the PPAN gene. The protein encoded by this gene is an evolutionarily conserved protein similar to yeast SSF1 as well as to the gene product of the Drosophila gene peter pan (ppan). SSF1 is known to be involved in the second step of mRNA splicing. Both SSF1 and ppan are essential for cell growth and proliferation. Exogenous expression of this gene was reported to reduce the anchorage-independent growth of some tumor cells. Read-through transcription of this gene with P2RY11/P2Y(11), an adjacent downstream gene that encodes an ATP receptor, has been found. These read-through transcripts are ubiquitously present and up-regulated during granulocyte differentiation.

• Description:

  Boster Bio Anti-PPAN Antibody Picoband® catalog # A10836-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  RNA-binding protein Nova-2; Astrocytic NOVA1-like RNA-binding protein; Neuro-oncological ventral antigen 2; NOVA2; ANOVA; NOVA3

• Gene Name:

  NOVA alternative splicing regulator 2

• Gene ID:

  56342

• UniProt:

  Q9NQ55

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human PPAN recombinant protein (Position: R20-E288).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Brain. Expression restricted to astrocytes.

• Applications:

  ELISA,Flow Cytometry,IF,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  May regulate RNA splicing or metabolism in a specific subset of developing neurons. Binds single strand RNA.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Communi, D., Suarez-Huerta, N., Dussossoy, D., Savi, P, Boeynaems, J.-M. Cotranscription and intergenic splicing of human P2Y11 and SSF1 genes. J. Biol. Chem. 276: 16561-16566, 2001. 2. Suarez-Huerta, N., Boeynaems, J.-M., Communi, D. Cloning, genomic organization, and tissue distribution of human Ssf-1. Biochem. Biophys. Res. Commun. 275: 37-42, 2000. 3. Welch, P. J., Marcusson, E. G., Li, Q.-X., Beger, C., Kruger, M., Zhou, C., Leavitt, M., Wong-Staal, F., Barber, J. R. Identification and validation of a gene involved in anchorage-independent cell growth control using a library of randomized hairpin ribozymes. Genomics 66: 274-283, 2000.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  29370 MW

• Observed Molecular Weight:

  65 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Nucleus.

• Protein Name:

  Aquaporin-6