Anti-beta Actin/ACTB Antibody Picoband®, HRP Conjugate

• Background:

  beta-Actin (ACTB) also known as BETA-ACTIN or ACTIN, CYTOPLASMIC, 1, is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Ueyama et al. (1996) used fluorescence in situ hybridization to map ACTB to 7p22. By PCR of somatic cell hybrid DNAs, they mapped 4 ACTB pseudogenes to other chromosomes. Karakozova et al. (2006) found that arginylation of beta-actin regulates cell motility. The majority of Ate1-null cells appeared smaller than wildtype cells and were apparently unable to form a lamella during movement along the substrate. Karakozova et al. (2006) concluded that arginylation of beta-actin apparently represents a critical step in the actin N-terminal processing needed for actin functioning in vivo.

• Description:

  Boster Bio Anti-beta Actin/ACTB Antibody catalog # PA1872. Tested in WB applications. This antibody reacts with Human, Monkey, Mouse, Rat, Chicken, Zebrafish. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Actin, cytoplasmic 1;Beta-actin;Actin, cytoplasmic 1, N-terminally processed;ACTB;

• Gene Name:

  Actin, cytoplasmic 1

• UniProt:

  P60709

• Host:

  Rabbit

• Reactivity:

  Chicken,Human,Monkey,Mouse,Rat,Zebrafish

• Cross Reactivity:

  No cross reactivity with other proteins

• Immunogen:

  A synthetic peptide corresponding to a sequence at the N-terminus of human beta Actin, identical to the related rat and mouse sequences.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Brain specific. .

• Applications:

  WB

• Purification:

  The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

• Concentration:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.

• Components:

  Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.

• References & Citations:

  1. Gunning, P., Ponte, P., Okayama, H., Engel, J., Blau, H., Kedes, L. Isolation and characterization of full-length cDNA clones for human alpha-, beta-, and gamma-actin mRNAs: skeletal but not cytoplasmic actins have an amino-terminal cysteine that is subsequently removed. Molec. Cell. Biol. 3: 787-795, 1983. 2. Karakozova, M., Kozak, M., Wong, C. C. L., Bailey, A. O., Yates, J. R, III, Mogilner, A., Zebroski, H., Kashina, A. Arginylation of beta-actin regulates actin cytoskeleton and cell motility. Science 313: 192-196, 2006. 3. Ueyama, H., Inazawa, J., Nishino, H., Ohkubo, I., Miwa, T. FISH localization of human cytoplasmic actin genes ACTB to 7p22 and ACTG1 to 17q25 and characterization of related pseudogenes.Cytogenet. Cell Genet. 74: 221-224, 1996.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  41737 MW

• Observed Molecular Weight:

  42 kDa

• Specificity:

  No cross reactivity with other proteins.

• Applications Notes:

  Western blot, 0.1-0.5μg/ml, Human, Monkey, Mouse, Rat, Chicken, Zebrafish
  

• Subcellular Location:

  Cytoplasm, cytoskeleton . Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

• Sequence Similarities:

  Belongs to the actin family.

• Protein Name:

  Actin, cytoplasmic 1