Anti-BST2/Tetherin Antibody Picoband®, HRP Conjugate

• Background:

  Tetherin, also known as bone marrow stromal antigen 2, is a lipid raft associated protein that in humans is encoded by the BST2 gene. Bone marrow stromal cells are involved in the growth and development of B-cells. The specific function of the protein encoded by the bone marrow stromal cell antigen 2 is undetermined; however, this protein may play a role in pre-B-cell growth and in rheumatoid arthritis.

• Description:

  Boster Bio Anti-BST2/Tetherin Antibody Picoband® catalog # A00934-3. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Tafazzin; Protein G4.5; TAZ; EFE2, G4.5

• Gene Name:

  tafazzin

• Gene ID:

  684

• UniProt:

  Q10589

• Host:

  Rabbit

• Reactivity:

  Human

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human BST2/Tetherin recombinant protein (Position: N49-S161).

• Clonality:

  Polyclonal

• Tissue Specificity:

  High levels in cardiac and skeletal muscle. Up to 10 isoforms can be present in different amounts in different tissues. Most isoforms are ubiquitous. Isoforms that lack the N-terminus are found in leukocytes and fibroblasts, but not in heart and skeletal muscle. Some forms appear restricted to cardiac and skeletal muscle or to leukocytes.

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Some isoforms may be involved in cardiolipin (CL) metabolism.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Cocka, L. J., Bates, P. Identification of alternatively translated tetherin isoforms with differing antiviral and signaling activities. PLoS Pathog. 8: e1002931, 2012. Note: Electronic Article. 2. Ishikawa, J., Kaisho, T., Tomizawa, H., Lee, B. O., Kobune, Y., Inazawa, J., Oritani, K., Itoh, M., Ochi, T., Ishihara, K., Hirano, T. Molecular cloning and chromosomal mapping of a bone marrow stromal cell surface gene, BST2, that may be involved in pre-B-cell growth. Genomics 26: 527-534, 1995. 3. Kuhl, A., Munch, J., Sauter, D., Bertram, S., Glowacka, I., Steffen, I., Sprecht, A., Hofmann, H., Schneider, H., Behrens, G., Pohlmann, S. Calcium-modulating cyclophilin ligand does not restrict retrovirus release. (Letter) Nature Med. 16: 155-157, 2010.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  50408 MW

• Observed Molecular Weight:

  25-45 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cytoplasm . Nucleus . Delocalizes from nucleus to cytoplasm when coexpressed with isoform Ets-1 p27. .

• Protein Name:

  Protein C-ets-1