Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband®, HRP Conjugate

• Background:

  The sigma-1 receptor (σ1R), one of two sigma receptor subtypes, is a chaperone protein at the endoplasmic reticulum (ER) that modulates calcium signaling through the IP3 receptor. In humans, the σ1 receptor is encoded by the SIGMAR1 gene. This gene encodes a receptor protein that interacts with a variety of psychotomimetic drugs, including cocaine and amphetamines. The receptor is believed to play an important role in the cellular functions of various tissues associated with the endocrine, immune, and nervous systems. As indicated by its previous name, opioid receptor sigma 1 (OPRS1), the product of this gene was erroneously thought to function as an opioid receptor; it is now thought to be a non-opioid receptor. Mutations in this gene has been associated with juvenile amyotrophic lateral sclerosis 16. Alternative splicing of this gene results in transcript variants encoding distinct isoforms.

• Description:

  Boster Bio Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband® catalog # A02493-2. Tested in ELISA, IHC, WB applications. This antibody reacts with Human, Monkey, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Heat shock protein beta-8;HspB8;Alpha-crystallin C chain;E2-induced gene 1 protein;Protein kinase H11;Small stress protein-like protein HSP22;HSPB8;CRYAC, E2IG1, HSP22;PP1629;

• Gene Name:

  Heat shock protein beta-8

• Gene ID:

  10280

• UniProt:

  Q99720

• Host:

  Rabbit

• Reactivity:

  Human,Monkey,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human Sigma1-receptor/SIGMAR1 recombinant protein (Position: G1-H154).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Predominantly expressed in skeletal muscle and heart. .

• Applications:

  WB,IHC,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Displays temperature-dependent chaperone activity.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. Al-Saif, A., Al-Mohanna, F., Bohlega, S. A mutation in sigma-1 receptor causes juvenile amyotrophic lateral sclerosis. Ann. Neurol. 70: 913-919, 2011. 2. Aydar, E., Palmer, C. P., Klyachko, V. A., Jackson, M. B. The sigma receptor as a ligand-regulated auxiliary potassium channel subunit. Neuron 34: 399-410, 2002. 3. Belzil, V. V., Daoud, H., Camu, W., Strong, M. J., Dion, P. A., Rouleau, G. A. Genetic analysis of SIGMAR1 as a cause of familial ALS with dementia. Europ. J. Hum. Genet. 21: 237-239, 2013.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Calculated Molecular Weight:

  21604 MW

• Observed Molecular Weight:

  25 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25 μg/ml, Human, Monkey, Mouse
  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Human, Mouse, Rat
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Cytoplasm . Nucleus . Translocates to nuclear foci during heat shock.

• Protein Name:

  Heat shock protein beta-8