Anti-GRB7 Antibody Picoband®, HRP Conjugate

• Background:

  GRB7, growth factor receptor-bound protein 7, is a protein which in humans is encoded by the GRB7 gene. The product of this gene belongs to a small family of adaptor proteins that are known to interact with a number of receptor tyrosine kinases and signaling molecules. This gene encodes a growth factor receptor-binding protein that interacts with epidermal growth factor receptor (EGFR) and ephrin receptors. The protein plays a role in the integrin signaling pathway and cell migration by binding with focal adhesion kinase (FAK). Alternative splicing results in multiple transcript variants encoding different isoforms, although the full-length natures of only two of the variants have been determined to date. GRB7 is an SH2-domain adaptor protein that binds to receptor tyrosine kinases and provides the intra-cellular link to the Ras proto-oncogene. Human GRB7 is located on the long arm of chromosome 17, next to the ERBB2 (alias HER2/neu) proto-oncogene. These two genes are commonly co-amplified (present in excess copies) in breast cancers. GRB7 though to be involved in migration, is well known to be over-expressed in testicular germ cell tumors, esophageal cancers, and gastric cancers.

• Description:

  Boster Bio Anti-GRB7 Antibody Picoband® catalog # A02528. Tested in ELISA, Flow Cytometry, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Natural killer cell receptor 2B4; Cd244; LOC685808

• Gene Name:

  CD244 molecule

• Gene ID:

  2886

• UniProt:

  Q14451

• Host:

  Rabbit

• Reactivity:

  Human,Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human GRB7 recombinant protein (Position: R29-S256).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Expressed in natural killer (NK) cells, T cells and dendritic cells.

• Applications:

  WB,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Modulate other receptor-ligand interactions to enhance leukocyte activation.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl and 0.2 mg Na2HPO4.

• References & Citations:

  1. Tanaka, S., Mori, M., Akiyoshi, T., Tanaka, Y., Mafune, K., Wands, J. R., Sugimachi, K.A novel variantof human Grb7 is associated with invasive esophageal carcinoma.J. Clin. Invest. 102: 821-827, 1998. 2. Vayssiere, B., Zalcman, G., Mahe, Y., Mirey, G., Ligensa, T., Weidner, K. M., Chardin, P., Camonis, J.Interaction of the Grb7 adapter protein with Rnd1, a new member of the Rho family.FEBS Lett. 467: 91-96, 2000.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  87164 MW

• Observed Molecular Weight:

  60 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  integral component of membrane

• Protein Name:

  Endothelin-converting enzyme 1