Anti-Cdc6 Antibody Picoband® (HRP Conjugate)

• Background:

  Cell division control ptotein 6 homolog, also called CDC18L or CDC6, is a protein that in humans is encoded by the CDC6 gene. By analysis of somatic cell hybrids and by fluorescence in situ hybridization, this gene is mapped to 17q21.2. The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Cdc6, a protein essential for the initiation of DNA replication. And this protein functions as a regulator at the early steps of DNA replication. It localizes in cell nucleus during cell cyle G1, but translocates to the cytoplasm at the start of S phase. The subcellular translocation of this protein during cell cyle is regulated through its phosphorylation by Cdks. Transcription of this protein was reported to be regulated in response to mitogenic signals through transcriptional control mechanism involving E2F proteins. This gene is involved in the initiation of DNA replication.

• Description:

  Boster Bio Anti-Cdc6 Antibody Picoband® catalog # PB9531. Tested in IHC, WB applications. This antibody reacts with Human, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Cell division control protein 6 homolog;CDC6-related protein;Cdc18-related protein;HsCdc18;p62 (cdc6);HsCDC6;CDC6;CDC18L;

• Gene Name:

  Cell division control protein 6 homolog

• UniProt:

  Q99741

• Host:

  Rabbit

• Reactivity:

  Human,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins

• Immunogen:

  E.coli-derived human Cdc6 recombinant protein (Position: V147-A549). Human Cdc6 shares 87.3% amino acid (aa) sequence identity with mouse Cdc6.

• Clonality:

  Polyclonal

• Tissue Specificity:

  Highly expressed in fetal lung, and kidney. In the adult, expression is mainly seen in lymphoid tissues, including spleen, thymus and peripheral blood lymphocytes.

• Applications:

  IHC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Involved in the initiation of DNA replication. Also participates in checkpoint controls that ensure DNA replication is completed before mitosis is initiated.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human, Rat, By Heat
  Western blot, 0.1-0.5μg/ml, Human, Rat
  

• Subcellular Location:

  Nucleus . Cytoplasm . The protein is nuclear in G1 and cytoplasmic in S-phase cells.

• Sequence Similarities:

  Belongs to the CDC6/cdc18 family.

• Protein Name:

  Cell division control protein 6 homolog