Anti-HCCS Antibody Picoband®, PE Conjugate

• Background:

  Cytochrome c-type heme lyase is an enzyme that in humans is encoded by the HCCS gene on chromosome X. The protein encoded by this gene is an enzyme that covalently links a heme group to the apoprotein of cytochrome c. Defects in this gene are a cause of microphthalmia syndromic type 7 (MCOPS7). Three transcript variants encoding the same protein have been found for this gene.

• Description:

  Boster Bio Anti-HCCS Antibody Picoband® catalog # A00924-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Human, Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Insulin-like growth factor-binding protein 1; IBP-1; IGF-binding protein 1; IGFBP-1; Igfbp1; Igfbp-1;

• Gene Name:

  insulin-like growth factor binding protein 1

• Gene ID:

  3052

• UniProt:

  P53701

• Host:

  Rabbit

• Reactivity:

  Human,Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human HCCS recombinant protein (Position: K76-S268).

• Clonality:

  Polyclonal

• Tissue Specificity:

  High expression in adult thyroid, lower expression in adult and fetal kidney and fetal brain. Not expressed in other tissues.

• Applications:

  WB,IHC,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration (By similarity). .

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

• References & Citations:

  1. Indrieri, A., Conte, I., Chesi, G., Romano, A., Quartararo, J., Tate, R., Ghezzi, D., Zeviani, M., Goffrini, P., Ferrero, I., Bovolenta, P., Franco, B. The impairment of HCCS leads to MLS syndrome by activating a non-canonical cell death pathway in the brain and eyes. EMBO Molec. Med. 5: 280-293, 2013. Note: Erratum: EMBO Molec. Med. 6: 849 only, 2014. 2. Morleo, M., Pramparo, T., Perone, L., Gregato, G., Le Caignec, C., Mueller, R. F., Ogata, T., Raas-Rothschild, A., de Blois, M. C., Wilson, L. C., Zaidman, G., Zuffardi, O., Ballabio, A., Franco, B. Microphthalmia with linear skin defects (MLS) syndrome: clinical, cytogenetic, and molecular characterization of 11 cases. Am. J. Med. Genet. 137A: 190-198, 2005. 3. Prakash, S. K., Cormier, T. A., McCall, A. E., Garcia, J. J., Sierra, R., Haupt, B., Zoghbi, H. Y., Van den Veyver, I. B. Loss of holocytochrome c-type synthetase causes the male lethality of X-linked dominant microphthalmia with linear skin defects (MLS) syndrome. Hum. Molec. Genet. 11: 3237-3248, 2002.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  27904 MW

• Observed Molecular Weight:

  31 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5μg/ml, Human, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Human, Rat
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Human
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Secreted.

• Protein Name:

  Insulin-like growth factor-binding protein 1