Anti-UHRF1 Antibody Picoband®, Dylight594 Conjugate

• Background:

  Ubiquitin-like, containing PHD and RING finger domains, 1 is a protein which in humans is encoded by the UHRF1 gene. This gene encodes a member of a subfamily of RING-finger type E3 ubiquitin ligases. The protein binds to specific DNA sequences, and recruits a histone deacetylase to regulate gene expression. Its expression peaks at late G1 phase and continues during G2 and M phases of the cell cycle. It plays a major role in the G1/S transition by regulating topoisomerase IIalpha and retinoblastoma gene expression, and functions in the p53-dependent DNA damage checkpoint. It is regarded as a hub protein for the integration of epigenetic information. This gene is up-regulated in various cancers, and it is therefore considered to be a therapeutic target. Multiple transcript variants encoding different isoforms have been found for this gene. A related pseudogene exists on chromosome 12.

• Description:

  Boster Bio Anti-UHRF1 Antibody Picoband® catalog # A01156-1. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  Troponin T, cardiac muscle; TnTc; Cardiac muscle troponin T; cTnT; TNNT2

• Gene Name:

  troponin T2, cardiac type

• Gene ID:

  29128

• UniProt:

  Q96T88

• Host:

  Rabbit

• Reactivity:

  Human,Mouse

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human UHRF1 recombinant protein (Position: K592-R793).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart.

• Applications:

  WB,ICC,IF,Flow Cytometry,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity.

• Components:

  Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.

• References & Citations:

  1. "Entrez Gene: UHRF1 ubiquitin-like, containing PHD and RING finger domains, 1". 2. Hopfner R, Mousli M, Jeltsch JM, Voulgaris A, Lutz Y, Marin C, Bellocq JP, Oudet P, Bronner C (Jan 2000)."ICBP90, a novel human CCAAT binding protein, involved in the regulation of topoisomerase IIalpha expression".Cancer Research 60 (1): 121–8.

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Observed Molecular Weight:

  100 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.25-0.5 μg/ml, Human, Mouse
  Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human
  Flow Cytometry (Fixed), 1-3 μg/1x10⁶ cells, Human
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Secreted.