Anti-CAMKIV/Camk4 Antibody Picoband®, Dylight594 Conjugate

• Background:

  Calcium/calmodulin-dependent protein kinase type IV is an enzyme that in humans is encoded by the CAMK4 gene. The product of this gene belongs to the serine/threonine protein kinase family, and to the Ca (2+)/calmodulin-dependent protein kinase subfamily. This enzyme is a multifunctional serine/threonine protein kinase with limited tissue distribution, that has been implicated in transcriptional regulation in lymphocytes, neurons and male germ cells.

• Description:

  Boster Bio Anti-CAMKIV/Camk4 Antibody Picoband® catalog # A01905-2. Tested in ELISA, Flow Cytometry, IF, IHC, ICC, WB applications. This antibody reacts with Mouse, Rat. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  C-C chemokine receptor type 1;C-C CKR-1;CC-CKR-1;CCR-1;CCR1;Macrophage inflammatory protein 1-alpha receptor;MIP-1alpha-R;RANTES-R;CD191;Ccr1;Cmkbr1;

• Gene Name:

  C-C chemokine receptor type 1

• Gene ID:

  12326

• UniProt:

  P08414

• Host:

  Rabbit

• Reactivity:

  Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived mouse Camk4 recombinant protein (Position: A19-Y469).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Detected in the heart, spleen, lung, peritoneal exudate cells and leukocytes.

• Applications:

  ELISA,Flow Cytometry,IF,IHC,ICC,WB

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Add 0.2ml of distilled water will yield a concentration of 500ug/ml.

• Function:

  Receptor for a C-C type chemokine. Binds to MIP-1-alpha, RANTES, and less efficiently, to MIP-1-beta or MCP-1 and subsequently transduces a signal by increasing the intracellular calcium ions level. Responsible for affecting stem cell proliferation.

• Components:

  Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4.

• References & Citations:

  1. Kang, H., Sun, L. D., Atkins, C. M., Soderling, T. R., Wilson, M. A., Tonegawa, S. An important role of neural activity-dependent CaMKIV signaling in the consolidation of long-term memory. Cell 106: 771-783, 2001. 2. Koga, T., Ichinose, K., Mizui, M., Crispin, J. C., Tsokos, G. C. Calcium/calmodulin-dependent protein kinase IV suppresses IL-2 production and regulatory T cell activity in lupus. J. Immun. 189: 3490-3496, 2012. 3. Raman, V., Blaeser, F., Ho, N., Engle, D. L., Williams, C. B., Chatila, T. A. Requirement for Ca(2+)/calmodulin-dependent kinase type IV/Gr in setting the thymocyte selection threshold. J. Immun. 167: 6270-6278, 2001.

• Storage Conditions:

  Store at -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freeze-thaw cycles.

• Calculated Molecular Weight:

  40895 MW

• Observed Molecular Weight:

  58 kDa

• Fragment:

  Rabbit IgG

• Applications Notes:

  Western blot, 0.1-0.25μg/ml, Mouse, Rat
  Immunohistochemistry (Paraffin-embedded Section), 2-5μg/ml, Mouse, Rat
  Immunocytochemistry/Immunofluorescence, 5μg/ml, Mouse
  Immunofluorescence, 5μg/ml, Rat
  Flow Cytometry (Fixed), 1-3μg/1x10⁶ cells, Mouse
  ELISA, 0.1-0.5μg/ml, -
  

• Subcellular Location:

  Cell membrane; Multi-pass membrane protein.