Anti-Dkk1 Antibody (Biotine Conjugate)

• Background:

  DKK1, Dickkopf-related protein 1, is a protein that in humans is encoded by the DKK1 gene. This gene encodes a protein that is a member of the dickkopf family. By genomic sequence analysis, determined that the DKK1 gene contains 4 exons. The DKK1 gene is mapped to 10q11.2 using FISH. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.

• Description:

  Boster Bio Anti-Dkk1 Antibody Picoband® catalog # A00632-3. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

• Synonyms:

  Dickkopf-related protein 1; Dickkopf-1; Dkk-1; hDkk-1; SK; DKK1; UNQ492/PRO1008

• Gene Name:

  dickkopf 1 homolog (Xenopus laevis)

• UniProt:

  O54908

• Host:

  Rabbit

• Reactivity:

  Mouse,Rat

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived mouse Dkk1 recombinant protein (Position: N73-H272).

• Clonality:

  Polyclonal

• Tissue Specificity:

  Placenta.

• Applications:

  IHC,ELISA

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6 (PubMed:22000856). DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease (PubMed:17143291). Inhibits the pro-apoptotic function of KREMEN1 in a Wnt-independent manner, and has anti- apoptotic activity (By similarity).

• Storage Conditions:

  At -20℃ for one year from date of receipt. After reconstitution, at 4℃ for one month. It can also be aliquotted and stored frozen at -20℃ for six months. Avoid repeated freezing and thawing.

• Fragment:

  Rabbit IgG

• Applications Notes:

  Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat
  ELISA, 0.1-0.5 μg/ml, -
  

• Subcellular Location:

  Secreted.