Anti-ATRIP Antibody Picoband®

• Background:

  This gene encodes an essential component of the DNA damage checkpoint. The encoded protein binds to single-stranded DNA coated with replication protein A. The protein also interacts with the ataxia telangiectasia and Rad3 related protein kinase, resulting in its accumulation at intranuclear foci induced by DNA damage. Multiple transcript variants encoding different isoforms have been found for this gene.

• Description:

  Boster Bio Anti-ATRIP Antibody Picoband® catalog # A03862-2. Tested in ELISA, Flow Cytometry, IF, ICC, WB applications. This antibody reacts with Human, Mouse, Rat, Monkey. The brand Picoband indicates this is a premium antibody that guarantees superior quality, high affinity, and strong signals with minimal background in Western blot applications. Only our best-performing antibodies are designated as Picoband, ensuring unmatched performance.

• Synonyms:

  AF4/FMR2 family member 4; ALL1-fused gene from chromosome 5q31 protein; Protein AF-5q31; Major CDK9 elongation factor-associated protein; AFF4; AF5Q31, MCEF; HSPC092

• Gene Name:

  AF4/FMR2 family member 4

• UniProt:

  Q8WXE1

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat, Monkey

• Cross Reactivity:

  No cross-reactivity with other proteins.

• Immunogen:

  E.coli-derived human ATRIP recombinant protein (Position: K109-D782) .

• Clonality:

  Polyclonal

• Tissue Specificity:

  Ubiquitously expressed. Strongly expressed in heart, placenta, skeletal muscle, pancreas and to a lower extent in brain. .

• Applications:

  WB, ICC, IF, Flow Cytometry, ELISA

• Field of Research:

  Epigenetics and Nuclear Signaling, Transcription, Cell Biology, Transcription Factors, Cell Cycle

• Purification:

  Immunogen affinity purified.

• Concentration:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Form:

  Lyophilized

• Reconstitution:

  Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.

• Function:

  Key component of the super elongation complex (SEC), a complex required to increase the catalytic rate of RNA polymerase II transcription by suppressing transient pausing by the polymerase at multiple sites along the DNA. In the SEC complex, AFF4 acts as a central scaffold that recruits other factors through interactions with ELL proteins (ELL, ELL2 or ELL3) and the P-TEFb complex. In case of infection by HIV-1 virus, the SEC complex is recruited by the viral Tat protein to stimulate viral gene expression. .

• Storage Conditions:

  At -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freezing and thawing.

• Fragment:

  Rabbit IgG

• Applications Notes:

  6

• Other Gene Names:

  ATRIP

• Subcellular Location:

  Nucleus . Associates to transcriptionally active chromatin but not at snRNA genes. .

• Protein Name:

  AF4/FMR2 family member 4