Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody

• Background:

  CD154 / CD40L (CD40 ligand) is a member of the tumor necrosis factor family, and is expressed primarily on activated CD4+ lymphocytes, but also on mast cells, basophils, eosinophils and human dendritic cells. Its counter-receptor CD40 is expressed on antigen presenting cells, including dendritic cells, macrophages, and B cells, and also on fibroblasts. Triggering of CD40 by CD40L causes maturation of dendritic cells and upregulation of antigen presentation in functions of the MHC and costimulatory molecules. CD40L also functions as a direct stimulating factor for T cells. CD40L plays also roles e.g. in antibody class switching and modulation of apoptosis in the germinal center.

• Description:

  Boster Bio Anti-Hu CD154 Purified Cd40lg Monoclonal Antibody (Catalog# M01114-2) . Tested in Flow Cytometry, ICC application (s) . This antibody reacts with Human, Primate.

• Synonyms:

  CD40L, CD40 ligand, TNFSF5, Ly62, gp39, T-BAM

• Gene Name:

  CD40LG

• UniProt:

  P27548

• Host:

  Mouse

• Reactivity:

  Human, Primate

• Immunogen:

  Human CD154 fusion protein. The mouse monoclonal antibody 24-31 detects an extracellular epitope of CD154 / CD40L (CD40-ligand), a 39 kDa cell surface type II glycoprotein expressed predominantly on activated CD4+ lymphocytes.

• Clonality:

  Monoclonal

• Clone:

  Clone: 24-31

• Tissue Specificity:

  Specifically expressed on activated CD4+ T- lymphocytes.

• Applications:

  Flow Cytometry, ICC

• Purification:

  Purified by protein-A affinity chromatography.

• Concentration:

  1 mg/ml

• Dilution:

  Flow cytometry: 2-6 μg/ml.

• Form:

  Liquid

• Function:

  Cytokine that binds to CD40/TNFRSF5. Costimulates T-cell proliferation and cytokine production. Its cross-linking on T- cells generates a costimulatory signal which enhances the production of IL4 and IL10 in conjunction with the TCR/CD3 ligation and CD28 costimulation. Induces the activation of NF- kappa-B and kinases MAPK8 and PAK2 in T-cells (By similarity) . Mediates B-cell proliferation in the absence of co-stimulus as well as IgE production in the presence of IL4. Involved in immunoglobulin class switching (PubMed:1374165) .

• Molecular Weight:

  29370 MW

• Shipping Conditions:

  Available

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Fragment:

  Mouse IgG1

• Other Gene Names:

  CD40 ligand

• Subcellular Location:

  Cell membrane

• Protein Name:

  CD40 ligand