Anti-RAB5C / RABL Rabbit Monoclonal Antibody

• Description:

  Boster Bio Anti-RAB5C / RABL Rabbit Monoclonal Antibody catalog # M05148-1. Tested in WB, IHC, ICC/IF applications. This antibody reacts with Human, Mouse, Rat.

• Synonyms:

  Caspase-14; CASP-14;3.4.22.-; Caspase-14 subunit p17, mature form; Caspase-14 subunit p10, mature form; Caspase-14 subunit p20, intermediate form; Caspase-14 subunit p8, intermediate form; CASP14

• Gene Name:

  Caspase-14

• UniProt:

  P51148

• Host:

  Rabbit

• Reactivity:

  Human, Mouse, Rat

• Immunogen:

  A synthesized peptide derived from human RAB5C / RABL

• Clonality:

  Monoclonal

• Clone:

  Clone: 28R55

• Tissue Specificity:

  Expressed in keratinocytes of adult skin suprabasal layers (from spinous layers to the stratum granulosum and stratum corneum) (at protein level) . Expressed in keratinocytes of hair shaft and sebaceous glands (at protein level) . In psoriatic skin only expressed at very low levels (PubMed:11175259) . The p17/10 mature form is expressed in epidermis stratum corneum, the p20/p8 intermediate form in epidermis upper granular cells of the stratum granulosum (PubMed:22825846) . .

• Applications:

  WB, IHC, ICC, IF

• Field of Research:

  Cell Biology, Intracellular

• Purification:

  Affinity-chromatography

• Concentration:

  Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.

• Form:

  Liquid

• Reconstitution:

  Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

• Function:

  Non-apoptotic caspase involved in epidermal differentiation. Is the predominant caspase in epidermal stratum corneum (PubMed:15556625) . Seems to play a role in keratinocyte differentiation and is required for cornification. Regulates maturation of the epidermis by proteolytically processing filaggrin (By similarity) . In vitro has a preference for the substate [WY]-X-X-D motif and is active on the synthetic caspase substrate WEHD-ACF (PubMed:16854378, PubMed:19960512) . Involved in processing of prosaposin in the epidermis (By similarity) . May be involved in retinal pigment epithelium cell barrier function (PubMed:25121097) . Involved in DNA degradation in differentiated keratinocytes probably by cleaving DFFA/ICAD leading to liberation of DFFB/CAD (PubMed:24743736) . .

• Storage Conditions:

  Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles.

• Fragment:

  IgG

• Applications Notes:

  WB 1:500-1:2000
  IHC 1:50-1:200
  ICC/IF 1:50-1:200

• Other Gene Names:

  RAB5C

• Subcellular Location:

  Cytoplasm . Nucleus.

• Protein Name:

  T-cell surface glycoprotein CD3 zeta chain