Recombinant Dechloromonas aromatica Chlorite Dismutase Protein (His Tag)

• Background:

  Chlorite dismutase (Cld) found in prokaryotic organisms, also known as Chlorite O2-lyase, is a b-type heme containing enzyme that catalyzes the reduction of chlorite into chloride plus dioxygen. The subunit of chlorite dismutase consists of a heme free N-terminal and a heme b containing C-terminal ferredoxin-like fold with high structural homology to the dye-decolorizing peroxidases (DyPs) . The physiological role of Cld in prokaryote has been shown that some microorganisms can use perchlorate or chlorate as terminal electron acceptors for anaerobic respiration thereby producing chlorite that must be detoxified. This enzyme has gained attention because it can be used in the development of bioremediation processes, biosensors, and controlled dioxygen production.

• Abbreviation:

  Chlorite O (2) -lyase; Chlorite Dismutase

• Synonyms:

  Chlorite O (2) -lyase; Chlorite dismutase; Cld; Daro_2580

• UniProt:

  Q47CX0

• Accession Number:

  Q47CX0

• Expression System:

  E.coli

• Tag:

  N-His

• Sequence:

  Met35-Asp282

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  31.3 kDa

• Observed Molecular Weight:

  32 kDa

• Species:

  Dechloromonas aromatica