Recombinant Mouse CD39/ENTPD1 Protein (His Tag)

• Background:

  Ectonucleoside triphosphate diphosphohydrolase-1 (NTPDase-1) is an integral membrane protein with an extracellular active site. Recombinant mouse NTPDase-1wasexpressed as a protein lacking its N- andC-terminaltransmembrane domains, resulting in the secretion of the soluble ectodomain. NTPDase-1was originally describedas CD39, a B lymphocyte cell surface marker. but it is also present on the surface of natural killer cells, T cells, and some endothelial cells. NTPDase1hydrolyzes the β-andγ phosphate residues of nucleotides, preferring ATP as the substrate. Through its hydrolysis of extracellular nucleotides, NTPDase-1plays arole in the regulation of purinergic signaling. NTPDase-1is involved in the processes of thromboregulation and vascular inflammation. The administration ofsoluble NTPDase-1may have therapeutic applications for the treatment of some vascular and transplantation-associateddiseases.

• Abbreviation:

  CD39; ENTPD1

• Synonyms:

  CD39; Ecto-ATP diphosphohydrolase 1; Ecto-ATPDase 1; Ecto-ATPase 1; Ecto-apyrase; Ectonucleoside triphosphate diphosphohydrolase 1; Lymphoid cell activation antigen; NTPDase 1

• UniProt:

  P55772

• Accession Number:

  P55772

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Thr38-Ile478

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  50.5 kDa

• Observed Molecular Weight:

  60-90 kDa

• Formulation:

  Supplied as a 0.2 μm filtered solution of 20mM Tris-HCl, 500mM NaCl, 10% Glycerol, pH 7.4.

• Species:

  Mouse