Recombinant Human CTLA4 Protein (GST Tag)

• Background:

  Cytotoxic Tlymphocyte 4 (CTLA-4; CD152) ; is a type I transmembrane T cell inhibitory molecule that is a member of the Ig superfamily. Human or mouse CTLA4 cDNA encodes 223 amino acids (aa) including a 35 aa signal sequence; a 126 aa extracellular domain (ECD) with one Ig-like V-type domain; a 21 aa transmembrane (TM) sequence; and a 41 aa cytoplasmic sequence.It is widely expressed with highest levels in lymphoid tissues. CD28 and CTLA-4; together with their ligands; B7-1 and B7-2; constitute one of the dominant costimulatory pathways that regulate T and B cell responses. CD28 and CTLA-4 are structurally homologous molecules that are members of the immunoglobulin (Ig) gene superfamily. CTLA4 transmits an inhibitory signal to T cells; whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T Cells and may play an important role in their functions. Tcell activation through the Tcell receptor and CD28 leads to increased expression of CTLA4.

• Abbreviation:

  CTLA4

• Synonyms:

  CD152; CTLA4; Cytotoxic T-Lymphocyte-Associated Protein 4; Cytotoxic T-lymphocyte protein 4; Cytotoxic T-lymphocyte-associated antigen 4

• UniProt:

  P16410

• Accession Number:

  P16410

• Expression System:

  HEK293 Cells

• Tag:

  C-GST

• Sequence:

  Lys36-Asp161

• Field of Research:

  Immunology; Stem cells

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  39.2 kDa

• Observed Molecular Weight:

  40-55 kDa

• Species:

  Human