Recombinant Human GDF5/BMP-14 Protein

• Background:

  Growth Differentiation Factor 5 (GDF-5, BMP-14) is a member of the BMP family of TGFβ superfamily proteins. Human GDF-5, -6, and -7 are a defined subgroup of the BMP family. GDF-5 is synthesized as a homodimeric precursor protein consisting of a 354 amino acid (aa) Nterminal proregion and a 120 aa C-terminal mature peptide. Mature human GDF-5 shares 99% aa sequence identity with both mature mouse and rat GDF-5. GDF-5 signaling is mediated by formation of a heterodimeric complex consisting of a type 1 (BMPR-IB) and a type II (BMPR-IIor Activin RII) serine/threonine kinase receptor which results in the phosphorylation and activation of cytosolic Smad proteins (Smad1, 5, and 8) . GDF-5 is involved in multiple developmental processes including limb generation, cartilage development, joint formation, bone morphogenesis, cell survival, and neuritogenesis. Inhibition of GDF-5 expression or alteration of its signaling can facilitate the development of osteoarthritis.

• Abbreviation:

  GDF5; BMP-14

• Synonyms:

  BMP-14; Bone morphogenetic protein 14; CDMP-1; CDMP1; Cartilage-derived morphogenetic protein 1; GDF-5; Growth/differentiation factor 5; LAP-4; LPS-associated protein 4; Lipopolysaccharide-associated protein 4; Radotermin

• UniProt:

  P43026

• Accession Number:

  P43026

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Ala382-Arg501

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cardiovascular; Stem cells

• Endotoxin:

  < 0.1 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  50

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  14.5 kDa

• Observed Molecular Weight:

  18 kDa

• Species:

  Human