Recombinant Human ST8SIA1 Protein (His Tag)

• Background:

  α-N-Acetylneuraminide α-2,8-Sialyltransferase (ST8SIA1) belongs to the glycosyltransferase 29 family. ST8SIA1 is a sialytransferase that catalyzes the transfer of sialic acid from CMP-sialic acid to GM3 to produce GD3 and GT3. ST8SIA1 is highly expressed in melanoma cell lines, adult and fetal brain, low expressed in adult and fetal lung. ST8SIA1 may act as a type II transmembrane protein with a short N-termianl cytoplasmic domain and a single-pass transmembrane domain folowed by an enzymatic domain in the lument of the Golgi apparatus.

• Abbreviation:

  ST8SIA1

• Synonyms:

  8-Sialyltransferase;8-Sialyltransferase 8A; Alpha-2; Alpha-N-Acetylneuraminide Alpha-2; Ganglioside GD3 Synthase; Ganglioside GT3 Synthase; SIAT8; SIAT8-A; SIAT8A; ST8SIA1; ST8SiaI; Sialyltransferase 8A; Sialytransferase St8Sia I

• UniProt:

  Q92185

• Accession Number:

  Q92185

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Sequence:

  Tyr49-Ser356

• Field of Research:

  Signal Transduction; Cancer; metabolism;

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Not Applicable

• Shipping Conditions:

  This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

• Storage Conditions:

  Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

• Calculated Molecular Weight:

  36.2 kDa

• Observed Molecular Weight:

  48 kDa

• Formulation:

  Supplied as a 0.2 µm filtered solution of 20mM Tris-HCl, 150mM NaCl, pH 8.0.

• Species:

  Human