Recombinant Human IGF-1/IGF1 Protein (aa 52-118)

• Background:

  Insulin-like growth factor I (IGF1) belongs to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGFs are generated by proteolytic processing of inactive precursor protein containing N-terminal and C-terminal propeptide regions. Mature human IGF-I consisting of 70 amino acids with 94% identity with mouse IGF1 and exhibits cross-species activity. IGF1 binds IGF-1R; IGF-2R; and the insulin receptor and plays a key role in cell cycle progression; cell proliferation and tumor progression. IGF1 expression is regulated by growth hormone.

• Abbreviation:

  IGF-1, IGF1

• Synonyms:

  IBP1; IGF-I; IGF1; Insulin-Like Growth Factor I; MGF; Mechano Growth Factor; Somatomedin-C

• UniProt:

  P05019

• Accession Number:

  P05019

• Expression System:

  E. coli

• Tag:

  None

• Sequence:

  Thr52-Ala118

• Applications:

  Cell culture

• Field of Research:

  Signal Transduction; Cancer; Developmental Biology;

• Endotoxin:

  < 0.5 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. The ED₅₀ for this effect is 20-100 ng/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  7.3 kDa

• Observed Molecular Weight:

  9 kDa

• Formulation:

  Lyophilized from a 0.2 µm filtered solution of 20mM NaAc-HAc, pH 4.5
  Normally 5% - 8% trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.
  Please refer to the specific buffer information in the printed manual.

• Species:

  Human