Recombinant Human CASP7/caspase 7 Protein (His Tag)

• Background:

  Caspase 7, also known as caspase-7 and MCH3, belongs to the cysteine-aspartic acid protease (caspase) family. Caspases play a role in the signal transduction pathways of apoptosis, necrosis and inflammation. There are two major classes of caspases: initiators and effectors. The initiator isoforms (caspases-1, -4, -5, -8, -9, -10, -11, -12) are activated by, and interact with, upstream adaptor molecules through protein-protein interaction domains known as CARD and DED. Effector caspases (-3, -6, -7) are responsible for cleaving downstream substrates and are sometimes referred to as the executioner caspases. Caspase 7 exists in lung, skeletal muscle, liver, kidney, spleen and heart, and moderately in testis. Caspase 7 cannot be detected in the brain. Caspase 7 functions in the activation cascade of caspases responsible for apoptosis execution. It cleaves and activates sterol regulatory element binding proteins (SREBPs) . It proteolytically cleaves poly (ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Overexpression promotes programmed cell death.

• Synonyms:

  CASP-7; CMH-1; ICE-LAP3; LICE2; MCH3

• UniProt:

  P55210

• Accession Number:

  P55210-1

• Expression System:

  E.coli

• Tag:

  C-His

• Sequence:

  Met 1-Gln 303

• Field of Research:

  Cell Biology, Cancer, Metabolism

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Not validated for activity

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  35.0 kDa

• Observed Molecular Weight:

  20 & 11 kDa

• Species:

  Human