Recombinant Human S100A12/CAGC Protein

• Background:

  S100 proteinis a family of low molecular weight protein found in vertebrates characterized by twoEF-hand calcium-binding motifs. There are at least 21 different S100 proteins, and the name is derived from the fact that the protein is100%soluble in ammonium sulfateat neutralpH. Most S100 proteins are disulfide-linked homodimer, and is normally present in cells derived from theneural crest, chondrocytes, macrophages, dendritic cells, etc. S100 proteins have been implicated in a variety of intracellular and extracellular functions. They are involved in regulation of protein phosphorylation, transcription factors, the dynamics of cytoskeleton constituents, enzyme activities, cell growth and differentiation, and the inflammatory response. Protein S100-A12, also known as S100 calcium-binding protein A12, Calcium-binding protein in amniotic fluid 1, Calgranulin-C, and S100A12, is a member of the S-101 family. Like the majority of S100 proteins, S100A12 is a dimer, with the interface between the two subunits being composed mostly of hydrophobic residues. The fold of S100A12 is similar to the other known crystal and solution structures of S100 proteins, except for the linker region between the two EF-hand motifs. S100A12 plays an important role in the inflammatory response.

• Synonyms:

  CGRP; Calcitermin; Calcium-binding protein in amniotic fluid 1; Calgranulin-C; EN-RAGE; Extracellular newly identified RAGE-binding protein; MRP-6; Migration inhibitory factor-related protein 6; Protein S100-A12; S100 calcium-binding protein A12; S100A12

• UniProt:

  P80511

• Accession Number:

  NP_005612.1

• Expression System:

  E.coli

• Tag:

  None

• Sequence:

  Met 1-Glu 92

• Applications:

  ELISA

• Field of Research:

  Signal Transduction; immunology

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 98 % as determined by reducing SDS-PAGE.

• Bioactivity:

  Immobilized recombinant human S100A12 at 2 μg/ml (100 μl/well) can bind human AGER with a linear range of 0.032-20 μg/ml.

• Reconstitution:

  Please refer to the printed manual for detailed information.

• Shipping Conditions:

  This product is provided as lyophilized powder which is shipped with ice packs.

• Storage Conditions:

  Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

• Calculated Molecular Weight:

  10.6 kDa

• Observed Molecular Weight:

  10 kDa

• Species:

  Human