Recombinant JNK Monoclonal Antibody

• Background:

  MAPK8 (Mitogen-activated protein kinase 8) is also named as JNK1, PRKM8, SAPK1, SAPK1C and belongs to the MAP kinase subfamily. MAPK8 is activated by dual phosphorylation at a Thr-Pro-Tyr motif during response to UV light. MAPK8 functions to phosphorylate c-Jun at N-terminal serine regulatory sites of Ser-63 and Ser-73, mapping within the transactivation domain. Phosphorylation of these sites in response to UV results in transcriptional activation of c-Jun. It has 4 isoforms produced by alternative splicing with the molecular weight of 46 kDa and 48 kDa. This protein can be phosphorylated and this antibody recognizes the 46 kDa and 55 kDa bands in western blot.

• Abbreviation:

  JNK

• UniProt:

  P45983

• Host:

  Rabbit

• Reactivity:

  Mouse; Rat

• Immunogen:

  Recombinant protein of human JNK1

• Target:

  AI849689; c Jun N terminal kinase 1; C-JUN kinase 1; c-Jun N-terminal kinase 1; EC 2.7.11.24; JNK 1; JNK; JNK-46; JNK1A2; JNK21B1/2; MAP kinase 8; MAPK 8; mapk8; Mitogen activated protein kinase 8; Mitogen-activated protein kinase 8; MK08; p54 gamma; Prkm8; Protein kinase JNK1; Protein kinase; mitogen-activated;8; SAPK 1; SAPK gamma; SAPK1; Stress-activated protein kinase 1; Stress-activated protein kinase JNK1

• Clonality:

  Monoclonal

• Clone:

  R08-6G2

• Conjugation:

  Unconjugated

• Applications:

  WB; IF

• Field of Research:

  Cancer; Immunology; Signal Transduction

• Purification:

  Affinity Purified

• Concentration:

  300 μg/mL

• Dilution:

  WB 1:500-1:1000; IF 1:50-1:100

• Buffer:

  50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 40% Glycerol, 0.01% Sodium azide and 0.05% protective protein

• Shipping Conditions:

  The product is shipped with ice pack, upon receipt, store it immediately at the temperature recommended.

• Storage Conditions:

  Store at -20°C Valid for 12 months. Avoid freeze / thaw cycles.

• Calculated Molecular Weight:

  48/53kDa

• Observed Molecular Weight:

  46/54kDa

• Isotype:

  IgG