Bradford Protein Colorimetric Assay Kit

• Target:

  TP

• Type:

  Colorimetric method

• Field of Research:

  Amino Acids and Proteins; Liver and Renal Function

• Assay Principle:

  Coomassie brilliant blue G-250 is red under the free state, and it has the maximum absorbance at 465 nm. When the Coomassie brilliant blue G-250 combined to protein, the compound will have the maximum at 595 nm. The absorbance value is directly proportional to the protein content, so the concentration of total protein can be calculated directly by measuring the OD value at 595 nm.

• Assay Performance Time:

  20 min

• Sample Type:

  Serum; plasma; animal tissue; cell

• Detection Range:

  0.046-0.6 mg/mL

• Sensitivity:

  0.046 mg/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C/room temperature for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

• Citation 01:

  https://www.jstage.jst.go.jp/article/jos/71/9/71_ess22033/_article/-char/ja/

• Inter CV:

  8.2%

• Intra CV:

  3.2%

• Detection Instrument:

  Microplate reader