H+K+-ATPase Activity Assay Kit

• Type:

  Colorimetric method

• Field of Research:

  Oxidative stress

• Assay Principle:

  ATPase can decompose ATP to produce ADP and inorganic phosphorus. The activity of ATPase can be expressed by measuring the production amount of inorganic phosphorus in unit time. The inorganic phosphorus reacts with ammonium molybdate in acidic solution to form ammonium molybdate compound, which is reduced with reducing agent to form molybdenum blue, and has absorption peak at 660 nm. Determine the concentration of molybdenum blue to calculate the amount of inorganic phosphorus.

• Assay Performance Time:

  60 min

• Sample Type:

  Animal tissue; cells

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.

• Citation 01:

  https://www.sciencedirect.com/science/article/pii/S0378517321011091

• Citation 02:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9709249/

• Citation 03:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9429807/

• Citation 04:

  https://europepmc.org/articles/pmc9424734/bin/table5.docx?javascript_support=no

• Citation 05:

  https://www.mdpi.com/1420-3049/27/16/5065

• Citation 06:

  https://www.mdpi.com/1420-3049/27/18/5919

• Citation 07:

  https://bmcpharmacoltoxicol.biomedcentral.com/articles/10.1186/s40360-022-00596-0

• Inter CV:

  9.8%

• Intra CV:

  4.4%

• Detection Instrument:

  Spectrophotometer