D-Lactic Acid/Lactate (LA) Colorimetric Assay Kit

• Target:

  D-LA

• Type:

  Colorimetric method

• Field of Research:

  Glycolysis and Lipid Metabolism

• Assay Principle:

  Using NAD+ as H+ receptor, D-lactate dehydrogenase (LDH) catalyzes the reaction of D-lactic acid and NAD+ to generate pyruvic acid and NADH respectively. NBT is reduced to a kind of purple compound during the reaction. Measure the OD value at 530 nm, and the concentration of D-lactic acid can be calculated.#N/A

• Assay Performance Time:

  20 min

• Sample Type:

  Serum; plasma; animal tissue; saliva sample

• Detection Range:

  0.06-8.0 mmol/L

• Sensitivity:

  0.06 mmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at 2~8°C for 12 months with shading light.

• Citation 01:

  https://microbiomejournal.biomedcentral.com/articles/10.1186/s40168-022-01390-0

• Citation 02:

  https://www.tandfonline.com/doi/abs/10.2147/IJN.S394544

• Citation 03:

  https://onlinelibrary.wiley.com/doi/abs/10.1111/cpr.13245

• Citation 04:

  https://www.mdpi.com/1999-4923/14/9/1759

• Citation 05:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207367/

• Citation 06:

  https://www.ncbi.nlm.nih.gov/pmc/articles/pmc8716831/

• Citation 07:

  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8977854/

• Citation 08:

  https://www.mdpi.com/1400748

• Citation 09:

  https://pubs.acs.org/doi/abs/10.1021/acs.jafc.2c04017

• Citation 10:

  https://onlinelibrary.wiley.com/doi/abs/10.1002/mnfr.202100253

• Citation 11:

  https://link.springer.com/article/10.1007/s12094-022-03008-1

• Citation 12:

  https://rep.bioscientifica.com/view/journals/rep/160/6/REP-20-0381.xml

• Inter CV:

  7.7%

• Intra CV:

  3.8%

• Detection Instrument:

  Microplate reader