Total Cholesterol and Cholesteryl Ester Fluorometric Assay Kit

• Type:

  Fluorometric method

• Field of Research:

  Glycolysis and Lipid Metabolism

• Assay Principle:

  Total Cholesterol (TC) includes free cholesterol (FC) and cholesteryl esters (CE) . Cholesterol ester can be hydrolyzed by cholesterol esterase to produce cholesterol and free fatty acid. Cholesterol is oxidized by cholesterol oxidase to produce △4-cholestenone and hydrogen peroxide. In the presence of the enzyme and probe, hydrogen peroxide can be catalyzed to produce the fluorescence substrate. The fluorescence intensity at the excitation wavelength of 535 nm and emission wavelength of 590 nm is proportional to the cholesterol concentration.

• Assay Performance Time:

  20 min

• Sample Type:

  Serum; plasma; animal tissue; cells

• Detection Range:

  0.12-30 μmol/L

• Sensitivity:

  0.12 μmol/L

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  This product can be stored at -20°C for 12 months with shading light.

• Citation 01:

  https://www.mdpi.com/1908036

• Inter CV:

  7.3%

• Intra CV:

  1.7%

• Detection Instrument:

  Fluorescence Microplate Reader