QuicKey Pro Monkey Cortisol ELISA Kit

• Abbreviation:

  Cortisol

• Cross Reactivity:

  This kit recognizes Monkey Cortisol in samples.No significant cross-reactivity or interference between Monkey Cortisol and analogues was observed

• Target:

  11β;17α;21-Trihydroxypregn-4-ene-3;20-dione

• Type:

  Competitive-ELISA

• Applications:

  ELISA

• Field of Research:

  Cell biology; Metabolism

• Assay Principle:

  This ELISA kit uses the Competitive-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with Monkey Cortisol. Samples (or Standards) and Horseradish Peroxidase (HRP) linked antibody specific for Monkey Cortisol are added to the micro ELISA plate wells. Monkey Cortisol in samples (or standards) competes with a fixed amount of Cortisol on the solid phase supporter for sites on the HRP linked detection antibody specific to Cortisol. Excess conjugate and unbound sample or standard are washed from the plate. The substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450±2 nm. The concentration of Monkey Cortisol in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  1 h 30 min

• Sample Type:

  Serum, plasma

• Detection Range:

  3.13-200 ng/mL

• Precision:

  Both intra-CV and inter-CV are < 10%.

• Recovery:

  80%-120%

• Sensitivity:

  1.13 ng/mL

• Shipping Conditions:

  Ice bag

• Storage Conditions:

  2-8°C,6 months

• Species:

  Monkey

• Recommended Usage:

  This ELISA kit applies to the in vitro quantitative determination of Monkey Cortisol concentrations in serum, plasma.