Unconjugated Human HLA-A*11:01&B2M Monomer Protein (Peptide free, MALS verified)

• Background:

  Human papillomavirus (HPV) is A kind of papillomavirus belonging to the milk polypoid virus family. It is a spherical DNA virus, which can cause the proliferation of squamous epithelium of human skin mucosa. HPV (human papillomavirus) for common warts, genital warts (condyloma acuminatum), and other symptoms. There are many types of human papillomavirus (HPV), with HPV 16 and 18 being high-risk types known to significantly increase the risk of cervical, vaginal and vulvar cancers in women and men. The PE-labeled human HLA-A*1101 HPV (IVCPICSQK) tetramer protein is a complex of HLA-A*1101 of the MHC Class I, B2M, and IVCPICSQK peptide of the HPV.

• Description:

  Unconjugated Human HLA-A*11:01&B2M Monomer Protein (HLM-H52H7) is expressed from human 293 cells (HEK293) . It contains AA Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M) (Accession # AAV53343.1 (HLA-A*11:01) & P61769 (B2M) ) .

• Specifications:

  This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™), and it is not biotinylated. The protein has a calculated MW of 36.0 kDa and 11.7 kDa. The protein migrates as 40-45 kDa and 12 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

• Accession Number:

  AAV53343.1 (HLA-A*11:01) & P61769 (B2M)

• Expression Region:

  Gly 25 - Thr 305 (HLA-A*11:01) & Ile 21 - Met 119 (B2M)

• Host:

  HEK293

• Target:

  HLA-A*1101 & B2M

• Conjugation:

  Unconjugated

• Tag:

  C-10xHis & C-Avi

• Source:

  Human

• Stability:

  ● -20°C to -70°C for 12 months in lyophilized state; ● -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.

• Endotoxin:

  1.0 EU per μg

• Purity:

  90%

• Bioactivity:

  Immobilized Unconjugated Human HLA-A*11:01&B2M Monomer Protein (Cat. No. HLM-H52H7) at 1 μg/mL (100 μL/well) can bind Anti-HLA class I Antibody, Human IgG1 (W6/32) with a linear range of 0.1-4 ng/mL (QC tested).

• Format:

  Powder

• Buffer:

  PBS, pH7.4

• Additives:

  Trehalose

• Molecular Weight:

  36.0 kDa and 11.7 kDa

• Shipping Conditions:

  RT

• Storage Conditions:

  -20°C

• Package Size:

  200ug*1

• Species:

  Human