Anti-TIAR Purified

Anti-TIAR Purified

• Background: TIAR is an ubiquitously expressed RNA-binding protein, which regulates translational control, splicing, and other activities, including apoptosis. TIAR attenuates CDK1 activity, and is essential for the G2/M checkpoint. It accumulates in nuclear foci in late G2 phase and prophase in cells under replication stress. In steady state TIAR shuttles between the cytoplasm and the nucleus, probably as a part of nucleocytoplasmic transport of mRNA, but under stress conditions it accumulates mRNA molecules in granules and prevents their translation. Nucleolytic activity of TIAR against attacked target cells of cytotoxic lymphocytes has also been reported. Similarly, e.g. in permeabilized thymocytes TIAR triggers DNA fragmentation.
• Specifications: The mouse monoclonal antibody 6E3 recognizes TIAR, an ubiquitously expressed approximately 42 kDa intracellular RNA-binding protein.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Mouse, Non-human primates
• Immunogen: GST-tagged recombinant human TIAR
• Target Antigen: TIAR
• Isotype: IgG2a κ
• Clone: 6E3
• Applications: Flow cytometry: Intracellular staining recommended dilution: 5 µg/mL
• Validated Applications: WB, IHC-P, ICC, FC, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Beck AR, Miller IJ, Anderson P, Streuli M: RNA-binding protein TIAR is essential for primordial germ cell development. Proc Natl Acad Sci U S A. 1998 Mar 395(5):2331-6., URL: https://pubmed.ncbi.nlm.nih.gov/9482885/,*Kedersha NL, Gupta M, Li W, Miller I, Anderson P: RNA-binding proteins TIA-1 and TIAR link the phosphorylation of eIF-2 alpha to the assembly of mammalian stress granules. J Cell Biol. 1999 Dec 27147(7):1431-42., URL: https://pubmed.ncbi.nlm.nih.gov/10613902/,*Piecyk M, Wax S, Beck AR, Kedersha N, Gupta M, Maritim B, Chen S, Gueydan C, Kruys V, Streuli M, Anderson P: TIA-1 is a translational silencer that selectively regulates the expression of TNF-alpha. EMBO J. 2000 Aug 119(15):4154-63., URL: https://pubmed.ncbi.nlm.nih.gov/10921895/,*Shukla S, Dirksen WP, Joyce KM, Le Guiner-Blanvillain C, Breathnach R, Fisher SA: TIA proteins are necessary but not sufficient for the tissue-specific splicing of the myosin phosphatase targeting subunit 1. J Biol Chem. 2004 Apr 2279(14):13668-76., URL: https://pubmed.ncbi.nlm.nih.gov/14736875/,
• Storage Conditions: Store at 2-8°C. Do not freeze.