Anti-Hu TCR Vdelta2 Purified

Anti-Hu TCR Vdelta2 Purified

• Background: TCR Vdelta2 is a variant of TCR delta chain, that is present on a major subset of human gamma/delta T cells. TCR Vgamma9/Vdelta2 (or Vgamma2/Vdelta2) T cells are able to recognize and kill various tumor cells, as this receptor heterodimer binds to certain phosphoantigens, expressed by tumors. They can recognize these antigens in an MHC-unrestricted manner. Similarly to NK cells, Vdelta2 T cells express MHC I receptors and killer Ig-like receptors, that are involved in tumor recognition and cytolysis. The potently cytotoxic subset of them is identified by cell surface expression of polysialyated CD56.
• Specifications: The mouse monoclonal antibody B6 recognizes an extracellular epitope of human TCR Vdelta2, which is expressed on the majority of gamma/delta T cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: human recombinant TCR Vdelta2
• Target Antigen: TCR Vdelta2
• Isotype: Igg1 κ
• Clone: B6
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Siegers GM, Felizardo TC, Mathieson AM, Kosaka Y, Wang XH, Medin JA, Keating A: Anti-leukemia activity of in vitro-expanded human gamma delta T cells in a xenogeneic Ph+ leukemia model. PLoS One. 2011 Feb 36(2):e16700., URL: https://pubmed.ncbi.nlm.nih.gov/21304898/,*Hunter S, Willcox CR, Davey MS, Kasatskaya SA, Jeffery HC, Chudakov DM, Oo YH, Willcox BE: Human liver infiltrating γδ T cells are composed of clonally expanded circulating and tissue-resident populations. J Hepatol. 2018 Sep69(3):654-665., URL: https://pubmed.ncbi.nlm.nih.gov/29758330/,*Ogongo P, Steyn AJ, Karim F, Kaylesh J Dullabh KJ, Awala I, Madansein R, Leslie A, Behar SM: Differential skewing of donor-unrestricted and γδ T cell repertoires in tuberculosis-infected human lungs. J Clin Invest. 2020 Jan 2130(1):214-230., URL: https://pubmed.ncbi.nlm.nih.gov/31763997/,*Li H, Luo K, Pauza CD: TNF-alpha is a positive regulatory factor for human Vgamma2 Vdelta2 T cells. J Immunol. 2008 Nov 15181(10):7131-7, URL: https://pubmed.ncbi.nlm.nih.gov/18981134/,*Fisher JP, Yan M, Heuijerjans J, Carter L, Abolhassani A, Frosch J, Wallace R, Flutter B, Capsomidis A, Hubank M, Klein N, Callard R, Gustafsson K, Anderson J: Neuroblastoma killing properties of Vδ2 and Vδ2-negative γδT cells following expansion by artificial antigen-presenting cells. Clin Cancer Res. 2014 Nov 1520(22):5720-32., URL: https://pubmed.ncbi.nlm.nih.gov/24893631/,*Davey MS, Willcox CR, Hunter S, KasatskayaSA, Remmerswaal EB, Salim M, Mohammed F, Bemelman FJ, Chudakov DM, Oo YH, Willcox BE: The human Vδ2 + T-cell compartment comprises distinct innate-like Vγ9 + and adaptive Vγ9 - subsets. Nat Commun. 2018 May 29(1):1760. , URL: https://pubmed.ncbi.nlm.nih.gov/29720665/,
• Storage Conditions: Store at 2-8°C. Do not freeze.