Anti-Hu PODXL Purified

Anti-Hu PODXL Purified

• Background: PODXL is a highly glycosylated sialomucin, which is expressed in many types of tumors, as well as it is a well known marker of embryonic stem cells. Overexpression of PODXL is an independent predictor of cancer progression, metastasis, and poor outcome. PODXL promotes tumor growts and invasiveness, and is a potential target for antibody therapy.
• Specifications: The mouse monoclonal antibody PcMab-47 recognizes a glycosylation-dependent epitope (aa 207-210) on human PODXL, a highly glycosylated transmembrane glycoprotein expressed above all in many types of cancer tissues, and on embryonic stem cells.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: recombinant PODXL ectodomain
• Target Antigen: PODXL
• Isotype: Igg1 κ
• Clone: PcMab-47
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: WB, FC, IHC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Schulten HJ, Hussein D: Array expression meta-analysis of cancer stem cell genes identifies upregulation of PODXL especially in DCC low expression meningiomas. PLoS One 2019 May 1314(5):e0215452. , URL: https://pubmed.ncbi.nlm.nih.gov/31083655/,*Itai S, Yamada S, Kaneko MK, Kato Y: Determination of critical epitope of PcMab-47 against human podocalyxin. Biochem Biophys Rep. 2018 Apr 2414:78-82., URL: https://pubmed.ncbi.nlm.nih.gov/29872738/,*Ogasawara S, Kaneko MK, Yamada S, Honma R, Nakamura T, Saidoh N, Yanaka M, Yoshida K, Fujii Y, Kato Y: PcMab-47: Novel antihuman podocalyxin monoclonal antibody for immunohistochemistry. Monoclon Antib Immunodiagn Immunother. 2017 Apr36(2):50-56., URL: https://pubmed.ncbi.nlm.nih.gov/28384052/,*Itai S, Ohishi T, Kaneko MK, Yamada S, Abe S, Nakamura T, Yanaka M, Chang YW, Ohba SI, Nishioka Y, Kawada M, Harada H, Kato Y: Anti-podocalyxin antibody exerts antitumor effects via antibody-dependent cellular cytotoxicity in mouse xenograft models of oral squamous cell carcinoma. Oncotarget 2018 Apr 279(32):22480-22497. , URL: https://pubmed.ncbi.nlm.nih.gov/29854293/,*Kaneko MK, Ohishi T, Kawada M, Kato Y: A cancer-specific anti-podocalyxin monoclonal antibody (60-mG 2a-f) exerts antitumor effects in mouse xenograft models of pancreatic carcinoma. Biochem Biophys Rep. 2020 Oct 1024:100826., URL: https://pubmed.ncbi.nlm.nih.gov/33088928/,
• Storage Conditions: Store at 2-8°C. Do not freeze.