Anti-Hu C3aR Purified

Anti-Hu C3aR Purified

• Background: C3aR is a 7TM transmembrane protein associated with G proteins, and serves as a receptor for C3a complement fragment. It is expressed mainly on neutrophils, mast cells, basophils, eosinophils, dendritic cells, monocytes, and macrophages. Upon detection of its ligand, the activated C3aR signaling cascade results in degranulation, superoxide production, and chemotaxis.
• Specifications: The mouse monoclonal antibody HC3aRZ8 recognizes an extracellular epitope of C3aR, a transmembrane chemotactic receptor for C3a anaphylatoxin.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: Human C3aR transfectants
• Target Antigen: C3aR
• Isotype: IgG2b
• Clone: HC3aRZ8
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL.
• Validated Applications: FC, IHC-F
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Abdelbaset-Ismail A, Borkowska-Rzeszotek S, Kubis E, Bujko K, Brzeźniakiewicz-Janus K, Bolkun L, Kloczko J, Moniuszko M, Basak GW, Wiktor-Jedrzejczak W, Ratajczak MZ: Activation of the complement cascade enhances motility of leukemic cells by downregulating expression of HO-1. Leukemia 2017 Feb31(2):446-458., URL: https://pubmed.ncbi.nlm.nih.gov/27451975/,*Larsen LF, Juel-Berg N, Hansen A, Hansen KS, Mills EN, van Ree R, Rådinger M, Poulsen LK, Jensen BM: No difference in human mast cells derived from peanut allergic versus non-allergic subjects. Immun Inflamm Dis. 2018 Dec6(4):416-427, URL: https://pubmed.ncbi.nlm.nih.gov/29992767/,*Reis ES, Chen H, Sfyroera G, Monk PN, Köhl J, Ricklin D, Lambris JD: C5a receptor-dependent cell activation by physiological concentrations of desarginated C5a: insights from a novel label-free cellular assay. J Immunol. 2012 Nov 15189(10):4797-805. , URL: https://pubmed.ncbi.nlm.nih.gov/23041570/,*Soruri A, Kiafard Z, Dettmer C, Riggert J, Köhl J, Zwirner J: IL-4 down-regulates anaphylatoxin receptors in monocytes and dendritic cells and impairs anaphylatoxin-induced migration in vivo. J Immunol. 2003 Mar 15170(6):3306-14., URL: https://pubmed.ncbi.nlm.nih.gov/12626590/,*Kiafard Z, Tschernig T, Schweyer S, Bley A, Neumann D, Zwirner J: Use of monoclonal antibodies to assess expression of anaphylatoxin receptors in tubular epithelial cells of human, murine and rat kidneys. Immunobiology 2007212(2):129-39., URL: https://pubmed.ncbi.nlm.nih.gov/17336833/,
• Storage Conditions: Store at 2-8°C. Do not freeze.