Anti-Hu C5aR2 Purified

Anti-Hu C5aR2 Purified

• Background: C5aR2, also known as C5L2, is one of two receptors for C5a (anaphylatoxin). It is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages, and seems to have both pro-inflammatory and anti-inflammatory roles, depending on circumstances. Unlike CD88, C5aR2 is not coupled to G-protein, thus the modulatory role is more likely.
• Specifications: The mouse monoclonal antibody 1D9-M12 recognizes an extracellular epitope on C5aR2 (C5L2), a C5a complement receptor, which is coexpressed with C5aR1 (CD88) in neutrophils, as well as e.g. in mast cells, astrocytes, or macrophages.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: L1.2 cells transfected with human C5aR2
• Target Antigen: C5aR2
• Isotype: IgG2a κ
• Clone: 1D9-M12
• Applications: Flow cytometry: Recommended dilution: 2-6 µg/mL
• Validated Applications: WB, FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Muenstermann M, Strobel L, Klos A, Wetsel RA, Woodruff TM, Köhl J, Johswich KO: Distinct roles of the anaphylatoxin receptors C3aR, C5aR1 and C5aR2 in experimental meningococcal infections. Virulence 2019 Dec10(1):677-694., URL: https://pubmed.ncbi.nlm.nih.gov/31274379/,*Fonseca MI, McGuire SO, Counts SE, Tenner AJ: Complement activation fragment C5a receptors, CD88 and C5L2, are associated with neurofibrillary pathology. J Neuroinflammation 2013, Feb 810:25, URL: https://pubmed.ncbi.nlm.nih.gov/23394121/,*An LL, Gorman JV, Stephens G, Swerdlow B, Warrener P, Bonnell J, Mustelin T, Fung M, Kolbeck R: Complement C5a induces PD-L1 expression and acts in synergy with LPS through Erk1/2 and JNK signaling pathways. Sci Rep. 2016 Sep 146:33346., URL: https://pubmed.ncbi.nlm.nih.gov/27624143/,*Spaan AN, Vrieling M, Wallet P, Badiou C, Reyes-Robles T, Ohneck EA, Benito Y, de Haas CJ, Day CJ, Jennings MP, Lina G, Vandenesch F, van Kessel KP, Torres VJ, van Strijp JA, Henry T: The staphylococcal toxins γ-haemolysin AB and CB differentially target phagocytes by employing specific chemokine receptors. Nat Commun. 2014 Nov 115:5438., URL: https://pubmed.ncbi.nlm.nih.gov/25384670/,*Hao J, Wang C, Yuan J, Chen M, Zhao MH: A pro-inflammatory role of C5L2 in C5a-primed neutrophils for ANCA-induced activation . PLoS One, 2013, Jun 138(6):e66305., URL: https://pubmed.ncbi.nlm.nih.gov/23785491/,
• Storage Conditions: Store at 2-8°C. Do not freeze.