Anti-Hu CD272 Purified

Anti-Hu CD272 Purified

• Background: CD272, a type I transmembrane glycoprotein, contains in its intracellular domain two ITIM sequences, which are upon CD272 triggering phosphorylated and recruit SHP phosphatases to attenuate cell activation. CD272 is expressed on B and T lymphocytes, NK cells, dendritic cells, and macrophages, and its ligand is CD270. Defects in CD272-CD270 inhibitory mechanism lead to autoimmune diseases. Overexpression of CD272 is a marker of tolerant T cells.
• Specifications: The mouse monoclonal antibody MIH26 recognizes an extracellular epitope of CD272, a transmembrane glycoprotein serving as a negative regulator of the activation in various leukocyte types.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human, Non-human primates
• Immunogen: cells transfected with human CD272
• Target Antigen: CD272
• Isotype: IgG2a κ
• Clone: MIH26
• Applications: Flow cytometry: Recommended dilution: 2-5 μg/mL.
• Validated Applications: FC
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Ramsay AG , Clear AJ, Fatah R, Gribben JG: Multiple inhibitory ligands induce impaired T-cell immunologic synapse function in chronic lymphocytic leukemia that can be blocked with lenalidomide: establishing a reversible immune evasion mechanism in human cancer. Blood. 2012 Aug 16120(7):1412-21., URL: https://pubmed.ncbi.nlm.nih.gov/22547582/?from_term=ramsay+ag+clear+aj&from_pos=4,*Zhang Z, Xu X, Lu J, Zhang S, Gu L, Fu J, Jin L, Li H, Zhao M, Zhang J, Wu H, Su L, Fu YX, Wang FS: B and T lymphocyte attenuator down-regulation by HIV-1 depends on type I interferon and contributes to T-cell hyperactivation. J Infect Dis. 2011 Jun 1203(11):1668-78., URL: https://pubmed.ncbi.nlm.nih.gov/21592997/?from_term=zhang+z+xu+x+gu+l+jin+l&from_pos=2,*Šedý JR, Balmert MO, Ware BC, Smith W, Němčovičová I, Norris PS, Miller BR, Aivazian D, Ware CF: A herpesvirus entry mediator mutein with selective agonist action for the inhibitory receptor B and T lymphocyte attenuator. J Biol Chem. 2017 Dec 22292(51):21060-21070., URL: https://pubmed.ncbi.nlm.nih.gov/29061848/?from_single_result=%C5%A1ed%C3%BD+jr+balmert+mo+ware+bc&expanded_search_query=%C5%A1ed%C3%BD+jr+balmert+mo+ware+bc,*Piancone F , Saresella M, Marventano I , La Rosa F , Zoppis M , Agostini S , Longhi R , Caputo D , Mendozzi L , Rovaris M , Clerici M: B lymphocytes in multiple sclerosis: Bregs and BTLA/CD272 expressing-CD19+ lymphocytes modulate disease severity. Sci Rep. 2016 Jul 146:29699. doi: 10.1038/srep29699., URL: https://pubmed.ncbi.nlm.nih.gov/27412504/,*Cairo C, Surendran N, Harris KM, Mazan-Mamczarz K, Sakoda Y, Diaz-Mendez F, Tamada K, Gartenhaus RB, Mann DL, Pauza CD: Vγ2Vδ2 T cell costimulation increases NK cell killing of monocyte-derived dendritic cells. Immunology. 2014 Sep 16144(3):422-430., URL: https://pubmed.ncbi.nlm.nih.gov/25227493/?from_single_result=cairo+c+surendran+n&expanded_search_query=cairo+c+surendran+n,
• Storage Conditions: Store at 2-8°C. Do not freeze.