Anti-Hu TdT Purified

Anti-Hu TdT Purified

• Background: Terminal deoxynucleotidyl transferase (TdT) is a template-independent DNA polymerase that catalyzes the addition of deoxynucleotides to the 3'-hydroxyl terminus of oligonucleotide primers. It is expressed in restricted populations of normal and malignant pre-B and pre-T lymphocytes during early differentiation, where it generates antigen receptor diversity by synthesizing non-germ line elements (N-regions) at the junctions of rearranged Ig heavy chain and T cell receptor gene segments. Alternatively spliced transcript variants encoding different isoforms of this protein have been described.
• Specifications: The mouse monoclonal antibody 41A recognizes TdT (terminal deoxynucleotidyl transferase an intracellular antigen), which is responsible for antigen receptor diversity in pre-B and pre-T lymphocytes.
• CAS Number: 9007-83-4
• Certification: RUO
• Host: Mouse
• Species Reactivity: Human
• Immunogen: His-TdT recombinant protein
• Target Antigen: TdT
• Isotype: IgG2a
• Clone: 41A
• Applications: Flow cytometry: Recommended dilution: 1-4 μg/mL. Intracellular staining.
• Validated Applications: FC, WB, IHC-P, ELISA
• Concentration: 1 mg/mL
• Format: Purified
• Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• References & Citations: *Montel-Hagen A, Seet CS, Li S, Chick B, Zhu Y, Chang P, Tsai S, Sun V, Lopez S, Chen HC, He C, Chin CJ, Casero D, Crooks GM: Organoid-Induced Differentiation of Conventional T Cells from Human Pluripotent Stem Cells. Cell Stem Cell. 2019 Mar 724(3):376-389.e8., URL: https://www.ncbi.nlm.nih.gov/pubmed/30661959,
• Storage Conditions: Store at 2-8°C. Do not freeze.