Mouse anti Human CD103, conjugated with PE

• Background:

  The CD103 antigen is a 160-130-105 kDa molecule which is expressed on mucosa-associated T lymphocytes and activated cells and on subset of TGF beta -1 cells. This Human intra epithelial lymphocyte marker is the E integrin chain associated with the b7 subunit. CD103 antibody stains a few bone marrow cells and, rarely, peripheral blood lymphocytes. CD103 is expressed by 0.5 to 2 % of resting lymphocytes in Peripheral blood and lymphoid organs.

• Synonyms:

  CD103 PE

• UniProt:

  P38570

• Host:

  Mouse

• Species Reactivity:

  Human

• Isotype:

  IgG1

• Clone:

  AX.14

• Conjugation:

  RPE

• Type:

  Single-Color Reagent

• Applications:

  Flow Cytometry

• Field of Research:

  Flow Cytometry

• Purification Method:

  Protein A/G Chromatography

• Assay Principle:

  PBMC: Add 10 µL of MAB/106 PBMC in 100 µL PBS. Mix gently and incubate for 15 minutes at 2° to 8° C. Wash twice with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. WHOLE BLOOD: Add 10 µL of MAB/100 µL of whole blood. Mix gently and incubate for 15 minutes at room temperature (20° C). Lyse the whole blood. Wash once with PBS and analyze or fix with 0.5% v/v of paraformaldehyde in PBS and analyze. See instrument manufacturers instructions for Lysed Whole Blood and Immunofluorescence analysis with a flow cytometer or microscope. ALLOPHYCOCYANIN: (APC) conjµgates are analyzed in multi-color flow cytometry with instruments equipped with a second laser and proper filters. Laser excitation is at 633 nm with a Helium Neon (HeNe) laser or a 600-640 nm (633 nm) range for a Dye laser. Peak fluorescence emission is at 660 nm.

• Stability:

  See expiration date on vial

• Concentration:

  Titered for flow cytometry

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide and 0,2% carrier protein

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Cepek K., Wong D. Brenner M., and Springer T., Leukocyte Typing V val. G p 1666-67 (1995).

• Shipping Conditions:

  Room Temperature

• Storage Conditions:

  Product should be stored at 4-8ºC; DO NOT FREEZE

• Functional Analysis:

  Flow Cytometry Staining

• CAS Number:

  9007-83-4