Alpha-Ketoglutarate Colorimetric/Fluorometric Assay Kit

• Description:

  α-Ketoglutarate (α-KG) is a key intermediate in the Krebs cycle, coming after isocitrate and before succinyl CoA. Anaplerotic reactions replenish the cycle by synthesizing α-KG from transamination of glutamate, or through the action of glutamate dehydrogenase. α-KG is an important nitrogen transporter. Being a key intermediate, it is one of the organic acids measured in newborns as an indicator of inborn errors of metabolism. BioVision's α-Ketoglutarate Assay Kit provides a simple, sensitive and rapid means for quantifying α-KG in a variety of samples. In the assay, α-ketoglutarate is transaminated with the generation of pyruvate which is utilized to convert a nearly colorless probe to both color (λmax = 570 nm) and fluorescence (Ex/Em = 535/587 nm). The α-Ketoglutarate Assay Kit is useful for detecting α-ketoglutarate in the range of 0.01 to 10 nmoles.

• Features & Benefits:

  • Simple procedure; takes ~ 30 minutes to complete the assay • Fast and convenient • Highly sensitive assays for measuring alpha-Ketaglutarate levels in biological samples

• Shipping Conditions:

  gel pack

• Storage Conditions:

  -20°C

• Shelf Life:

  12 months