Cell Cytotoxicity Assay Kit II (WST-SRB-Crystal Violet)

Cytotoxicity assays are essential tools in drug discovery. In vitro single parameter cytotoxicity assays, such as using tetrazolium salt (WST), sulforhodamine B (SRB) or crystal violet (CV) are widely used in cultured cellsin order to assess the cytotoxicity of potential drug candidates as these methods are fast, reproducible, and do no require using living mammals during early drug discovery stages. However, the most critical problem of using single parameter assays is the determination of their IC50 data offers a limited perspective and can lead to erroneous assumptions or erroneous, unsucessful results when the selected candidates are used in more complex models (false positives). On the other hand, if the cytotoxicity assay is not selected appropriately, false negative compounds that are actually cytotoxic may be easily missed. Thus, the analysis of candidates as potential cytotoxic compounds is usually more comprehensive when two or more cytotoxicity assays are performed. Additionally, SRB, WST and CV cyototoxic assays offer relatively inexpensive, accurate, yet reliable results in a timely manner. BioVision’s WST-SRB-Crystal Violet Combined Cytotoxicity Assay Kit allow researchers to test the cytotoxicity effect of their candidate compounds in different biological processes: cell proliferation (WST), protein synthesis (SRB) and/or total DNA synthesis (CV). The assays are compatible to each other and same set of cultured cells can be tested using these assays. The kit is simple, fast, high-throughput compatible and provides an efficient research tool for the quantitative measurement of cell cytotoxicity in terms of viability, protein and DNA synthesis

Eukaryotes

The assay measures cell cytotoxicity in response to synthetic compounds and can be adapted for HTS applications

Absorbance [OD: WST/NR/CV: 450 /565/570 nm

• Simple procedures; take less than 4 hours • Convenient • Uses a water-soluble tetrazolium salt WST reagent, SRB and Crystal Violet . Method are simple and do not require extensive washing/harvesting/or solubilization steps. The entire assay can be performed directly in a 96-well plate.

gel pack

-20°C