pCR-BluntII-TOPO-RORA Plasmid

  • Catalog number
    PVTB01095-1
  • Price
    385.05 USD
  • Size
    2 ug
  • Group
    PCR, polymerase chain reaction
  • About
    TAQ or Pfu or Pfx or other enzymes are used for polycmerase chain reaction and have different specificity. The mores specific the lower the yield.
  • Properties
    Thermocyclers can be callibrated for identical ramping curves to obtain a more accurate PCR.
  • Kit
    Plasmid mini made and maxi DNA purification kits can be silica gel or anion exchange, endotoxin free and are used to produce pure plasmids that are small DNA molecules within a cell separated from chromosomal DNA and can replicate independently. They are most commonly found in bacteria as small circular, double-stranded DNA molecules; however, plasmids are sometimes present in archaea and eukaryotic organisms. In nature, plasmids often carry genes that may benefit the survival of the organism, for example antibiotic resistance. While the chromosomes are big and contain all the essential information for living, plasmids usually are very small and contain only additional information. Artificial plasmids are widely used as vectors in molecular cloning, serving to drive the replication of recombinant DNA sequences within host organisms.
  • Gene target
  • Gene symbol
    RORA-AS1, RORA-AS2, RORA
  • Short name
    pCR-BluntII-TOPO-RORA Plasmid
  • Technique
    plasmid, PCR, The polymerase chain reaction (PCR) amplifies the DNA in your sample. For real time PCR the cycle threshold Ct values willneed to be set before the experiment. Than the RT-PCR starts from RNA and real time PCR quantitates the cDNA so the RNA in the sample on given time of the experiment. plasmids in 1
  • Alternative name
    PCR test kit-BluntII-TOPO-RAR-related orphan receptor A Plasmid
  • Alternative technique
    plasmids, dna-amplification
  • Alternative to gene target
    RAR-related orphan receptor A, NR1F1 and ROR1 and ROR2 and ROR3 and RZR-ALPHA and RZRA, RORA and IDBG-14920 and ENSG00000069667 and 6095, direct ligand regulated sequence-specific DNA binding transcription factor activity, nuclei, Rora and IDBG-181478 and ENSMUSG00000032238 and 19883, RORA and IDBG-646570 and ENSBTAG00000015904 and 535597,790889
Gene info
Gene info
Gene info
MeSH Data
  • Name
  • Concept
    Scope note: In vitro method for producing large amounts of specific DNA or RNA fragments of defined length and sequence from small amounts of short oligonucleotide flanking sequences (primers). The essential steps include thermal denaturation of the double-stranded target molecules, annealing of the primers to their complementary sequences, and extension of the annealed primers by enzymatic synthesis with DNA polymerase. The reaction is efficient, specific, and extremely sensitive. Uses for the reaction include disease diagnosis, detection of difficult-to-isolate pathogens, mutation analysis, genetic testing, DNA sequencing, and analyzing evolutionary relationships.
  • Tree numbers
    • E05.393.620.500
  • Qualifiers
    ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data
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