XRCC1 Antibody

• Catalog number
  R30722
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  XRCC1
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P, ICC
• Recommended dilutions
  Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml,Immunocytochemistry: 0.5-1ug/ml
• Notes
  The stated application concentrations are suggested starting amounts. Titration of the XRCC1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This XRCC1 antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P18887
• Purity
  Antigen affinity
• Description
  X-ray repair, complementing defective, in chinese hamster 1 is a DNA repair protein which complexes with DNA ligase III. The protein encoded by this gene is involved in the efficient repair of DNA single-strand breaks formed by exposure to ionizing radiation and alkylating agents. The XRCC1 gene is mapped to 19q13.31. The protein interacts with DNA ligase III, polymerase beta and poly(ADP-ribose) polymerase to participate in the base excision repair pathway. It may play a role in DNA processing during meiogenesis and recombination in germ cells. A rare microsatellite polymorphism in this gene is associated with cancer in patients of varying radiosensitivity. XRCC1 is phosphorylated in vivo and in vitro by CK2, and CK2 phosphorylation of ser518, thr519, and thr523 largely determines aprataxin binding to XRCC1 through its FHA domain.
• Immunogen
  An amino acid sequence from the N-terminus of human XRCC1 (QDSTHCAENLLKADTYRKWR) was used as the immunogen for this XRCC1 antibody (100% mouse homology).
• Storage
  After reconstitution, the XRCC1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  XRCC1  
• Gene symbol
  XRCC1
• Short name
  Anti-XRCC1
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to XRCC1
• Alternative technique
  antibodies
• Alternative to gene target
  X-ray repair complementing defective repair in Chinese hamster cells 1, RCC, XRCC1 and IDBG-55268 and ENSG00000073050 and 7515, protein binding, nuclei, Xrcc1 and IDBG-155000 and ENSMUSG00000051768 and 22594, XRCC1 and IDBG-639023 and ENSBTAG00000016268 and 616905

Gene info

• Identity
  HGNC:12828
• Gene
  XRCC1
• Long gene name
  X-ray repair cross complementing 1
• Synonyms gene
  • RCC
• Synonyms gene name
  • X-ray repair complementing defective repair in Chinese hamster cells 1
• GenBank acession
  • M36089
• Locus
  19q13.31
• Discovery year
  2001-06-22
• Entrez gene record
  7515
• Pubmed identfication
  • 2247054
• RefSeq identity
  • NM_006297
• VEGA ID
  OTTHUMG00000182699
• Locus Specific Databases
  • LRG_784

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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