Vimentin, Human Recombinant

• Catalog number
  P1012-250
• Price
  Please ask
• Size
  250 ug

• Synonyms
  VIM
• Alternative_names
  VIM
• Description
  A class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells.
• Recombinant
  Yes
• Source
  E. coli
• Purity by SDS PAGE
  ≥95%
• Assay
  SDS-PAGE
• Purity
  ≥95%
• Endotoxin Level
  < 1 EU/ug
• Molecular Weight
  54.3 kDa
• Storage Temp
  -20°C
• Shipping
  Gel pack
• Shelf Life
  12 months
• Appearance
  Dry powder
• Physical form description
  Lyophilized powder
• Reconstitution Instructions
  Reconstitute in sterile deionized water to a concentration of 0.1-1.0 mg/ml. Do not vortex. Additional carrier protein (example 0.1% BSA) is recommended for long term storage.
• Background Information
  Vimentins are class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is attached to the nucleus, endoplasmic reticulum, and mitochondria, either laterally or terminally. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
• Amino acid sequence
  Full length of human Vimentin with a 6-residue C-terminal His-Tag
• Handling
  Centrifuge the vial prior to opening.
• Usage
  For Research Use Only! Not to be used in humans
• Datasheet
• Properties
  Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.
• Additional source
  Recombinants or rec. proteins
• Group
  recombinants

• Gene target
  Vimentin  
• Gene symbol
  VIM2P, VIM, VMAC
• Short name
  Vimentin, Recombinant
• Technique
  Recombinant, E. coli recombinant proteins are genetic recombinations in Escherichia coli, supplied as white sterile powder lyopillized. Biovision advises they will be reconstituted in a buffer soluion or culture medium for cell culture.
• Species
  Human, Humans
• Alternative name
  Vimentin, H. sapiens Rec.
• Alternative technique
  rec

Gene info

• Identity
  HGNC:43891
• Gene
  VIM2P
• Long gene name
  vimentin 2, pseudogene
• Synonyms gene
  • VIMP1
• Synonyms gene name
  • vimentin pseudogene 1
  • vimentin 2, pseudogene (functional)
• Synonyms
  • lncRNA-CIR
  • RP11-162L10.1
  • VIM2
• Synonyms name
  • long non-coding RNA, cartilage injury-related
• Locus
  6q22.32
• Discovery year
  2012-04-23
• Entrez gene record
  100130535
• Pubmed identfication
  • 24757148
  • 30292414
• RefSeq identity
  • NG_022326
• VEGA ID
  OTTHUMG00000015519

Gene info

• Identity
  HGNC:12692
• Gene
  VIM
• Long gene name
  vimentin
• GenBank acession
  • M14144
• Locus
  10p13
• Discovery year
  2001-06-22
• Entrez gene record
  7431
• RefSeq identity
  • NM_003380
• Classification
  • Intermediate filaments Type III
• VEGA ID
  OTTHUMG00000017744

Gene info

• Identity
  HGNC:33803
• Gene
  VMAC
• Long gene name
  vimentin type intermediate filament associated coiled-coil protein
• GenBank acession
  • BC110802
• Locus
  19p13.3
• Discovery year
  2009-06-12
• Entrez gene record
  400673
• Pubmed identfication
  • 14985129
• RefSeq identity
  • NM_001017921
• VEGA ID
  OTTHUMG00000180618

MeSH Data

• Name
  Primary Cell Culture
• Concept
  Scope note: The initial culturing of cells derived directly from fresh TISSUES.
• Tree numbers
  • E01.370.225.500.223.500
  • E05.200.500.265.500
  • E05.242.223.500
  • E05.481.500.249.500
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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