VHL Antibody

• Catalog number
  F49682-0.4ML
• Price
  Please ask
• Size
  0.4 ml

• Category
  Antibody
• Concentration
  In 1X PBS, pH 7.4, with 0.09% sodium azide
• Form
  Purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  VHL
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  IHC, FACS, WB, ELISA
• Recommended dilutions
  Western blot: 1:1000,IHC (Paraffin): 1:50-1:100,Flow Cytometry: 1:10-1:50
• Notes
  Titration of the VHL antibody may be required due to differences in protocols and secondary/substrate sensitivity.
• Intented use
  This VHL antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P40337
• Purity
  Purified
• Description
  Von Hippel-Lindau syndrome (VHL) is a dominantly inherited familial cancer syndrome predisposing to a variety of malignant and benign tumors. A germline mutation of VHL gene is the basis of familial inheritance of VHL syndrome. The protein is a component of the protein complex that includes elongin B, elongin C, and cullin-2, and possesses ubiquitin ligase E3 activity. This protein is involved in the ubiquitination and degradation of hypoxia-inducible-factor (HIF), which is a transcription factor that plays a central role in the regulation of gene expression by oxygen. RNA polymerase II subunit POLR2G/RPB7 is also reported to be a target of this protein.
• Immunogen
  A portion of amino acids 181-210 from the human protein was used as the immunogen for this VHL antibody.
• Storage
  Aliquot the VHL antibody and store frozen at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized.
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  VHL  
• Gene symbol
  VHL
• Short name
  Anti-VHL
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit Ig
• Alternative name
  Antibodies to VHL
• Alternative technique
  antibodies
• Alternative to gene target
  von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase, HRCA1 and pVHL and RCA1 and VHL1, VHL and IDBG-17819 and ENSG00000134086 and 7428, enzyme binding, nuclei, Vhl and IDBG-178105 and ENSMUSG00000033933 and 22346, BT.79817 and IDBG-644624 and ENSBTAG00000016101 and 540957

Gene info

• Identity
  HGNC:12687
• Gene
  VHL
• Long gene name
  von Hippel-Lindau tumor suppressor
• Synonyms gene name
  • von Hippel-Lindau syndrome
  • von Hippel-Lindau tumor suppressor, E3 ubiquitin protein ligase
• Synonyms
  • VHL1
• GenBank acession
  • L15409
• Locus
  3p25.3
• Discovery year
  1986-01-01
• Entrez gene record
  7428
• Pubmed identfication
  • 9671762
• RefSeq identity
  • NM_000551
• VEGA ID
  OTTHUMG00000128668
• Locus Specific Databases
  • UMD Locus Specific Databases
  • LRG_322

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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