Versican Antibody

• Catalog number
  R31959
• Price
  Please ask
• Size
  0.1mg

• Category
  Antibody
• Concentration
  0.5mg/ml if reconstituted with 0.2ml sterile DI water
• Form
  Antigen affinity purified
• Conjugation
  Unconjugated
• Clone
  Polyclonal antibody
• Recognised antigen
  Versican
• Host animal
  Rabbit (Oryctolagus cuniculus)
• Clonality
  Polyclonal (rabbit origin)
• Species reactivity
  Human (Homo sapiens), Mouse (Mus musculus), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.
• Tested applications
  WB, IHC-P
• Recommended dilutions
  Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml
• Notes
  Optimal dilution of the Versican antibody should be determined by the researcher.
• Intented use
  This Versican antibodyis to be used only for research purposes and not for diagnostics..
• Uniprot
  P13611
• Purity
  Antigen affinity
• Description
  Versican (VCAN), also known as CSPG2, is a large extracellular matrix proteoglycan that is present in a variety of human tissues. Versican is a large chondroitin sulfate proteoglycan. The Versican gene is mapped on 5q14.2-q14.3. The human versican gene contains 15 exons spanning more than 90 kb. The distribution of versican by using affinity-purified polyclonal antibodies that recognize the core protein of the prominent versican splice variants V0 and V1. Versican staining was noted in the central and peripheral nervous system, in the basal layer of the epidermis, and on the luminal surface of some glandular epithelia. Biochemical purification of LLC-conditioned medium led to identification of the extracellular matrix proteoglycan versican, which is upregulated in many human tumors including lung cancer, as a macrophage activator that acts through TLR2 and its coreceptors TLR6 and CD14. By activating TLR2:TLR6 complexes and inducing TNF-alpha secretion by myeloid cells, versican strongly enhances LLC metastatic growth.
• Immunogen
  Amino acids KWSKIEVDKNGKDLKETTVLVAQNGNIK of human Versican were used as the immunogen for the Versican antibody.
• Storage
  After reconstitution, the Versican antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.
• Localization
  Intracellular, secreted
• Properties
  If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
• French translation
  anticorps

• Gene target
  Versican  
• Gene symbol
  VCAN
• Short name
  Anti-Versican
• Technique
  Antibody, antibodies against human proteins, antibodies for
• Isotype
  Rabbit IgG
• Alternative name
  Antibodies to Versican
• Alternative technique
  antibodies

Gene info

• Identity
  HGNC:2464
• Gene
  VCAN
• Long gene name
  versican
• Synonyms gene
  • CSPG2
• Synonyms gene name
  • chondroitin sulfate proteoglycan 2
• Synonyms
  • PG-M
• Synonyms name
  • versican proteoglycan
• GenBank acession
  • X15998
• Locus
  5q14.2-q14.3
• Discovery year
  1991-07-18
• Entrez gene record
  1462
• Pubmed identfication
  • 1478664
  • 21063030
• RefSeq identity
  • NM_004385
• Classification
  • C-type lectin domain containing
  • Sushi domain containing
  • Hyalectan proteoglycans
  • V-set domain containing
• VEGA ID
  OTTHUMG00000131321

MeSH Data

• Name
  Blotting, Western
• Concept
  Scope note: Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
• Tree numbers
  • E05.196.401.143
  • E05.301.300.096
  • E05.478.566.320.200
  • E05.601.262
  • E05.601.470.320.200
• Qualifiers
  ethics, trends, veterinary, history, classification, economics, instrumentation, methods, standards, statistics & numerical data

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