VeriScript Reverse Transcriptase

VeriScript Reverse Transcriptase is available 1 time from Biovision labs

9224-1000 | VeriScript Reverse Transcriptasesize: 1000 UN | 158.76 USD

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  • Background
    VeriScript Reverse Transcriptase is an engineered version of M-MLV Reverse Transcriptase with reduced RNase H activity and increased thermostability, allowing for first-strand cDNA synthesis with more full length cDNAs up to 12.5 kb. This DNA polymerase can synthesize complementary DNA from single-stranded DNA, RNA, or a RNA:DNA hybrid.
  • Concentration
    N/A
  • Molecular weight
    N/A
  • Synonym
    Verscript
  • Other name
    Verscript
  • Recombinant
    Yes
  • Source
    Recombinant
  • Purification
    ≥95%
  • Tested applications
    SDS-PAGE, N/A
  • Level of endotoxin
    N/A
  • Tested activity
    200 unit/μl
  • Biological activity
    N/A
  • Reesults
    N/A
  • Binding ability
    N/A
  • Unit
    One unit is the amount of enzyme activity that incorporates 1 nmole of dTTP into acid insoluble material in 10 minutes at 37°C using poly(rA)-oligo(dT)18 as template-primer.
  • Storage condition
    -20°C
  • Shipping under
    Gel pack
  • Physical appearance
    Liquid
  • Physical properties
    Recombinant VeriScript Reverse Transcriptase in 20mM Tris-HCl, pH 7.5, 100 mM NaCl, 0.1 mM EDTA, 1 mM DTT, 0.01% Igepal CA-630, and 50% Glycerol.
  • How to reconstitute
    N/A
  • Aa sequence
    N/A
  • Before use
    Centrifuge the vial prior to opening.
  • Notes
    For Research Use Only! Not to be used in humans
  • Description
    Reverse transcription primers are used in PCR but in vivo reverse transcription begins when the viral particle that enters the cytoplasm of a target cell with its reverse transcriptase. The viral RNA genome enters the cytoplasm as part of a nucleoprotein complex that has not been well characterized. The process of reverse transcription generates, in the cytoplasm, a linear DNA via an intricate series of steps. This DNA is collinear with its RNA template, but it contains terminal duplications known as the long terminal repeats (LTRs) that are not present in viral RNA . Extant models for reverse transcription propose that two specialized template switches known as strand-transfer reactions or “jumps” are required to generate the LTRs.
  • Goup
    reverse transcription
  • Gene target
  • Short name
    VeriScript Reverse Transcriptase
  • Technique
    reverse
  • Alternative name
    VeriScript Reverse Transcriptase
  • Alternative technique
    transctription
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